diff --git a/.github/workflows/github-actions-ci.yml b/.github/workflows/github-actions-ci.yml
index e18c8c2d..e533a1b4 100644
--- a/.github/workflows/github-actions-ci.yml
+++ b/.github/workflows/github-actions-ci.yml
@@ -20,7 +20,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ["3.9", "3.10", "3.11", "3.12", "3.13", "3.14-dev"]
+        python-version: ["3.10", "3.11", "3.12", "3.13", "3.14"]
 
     steps:
       - uses: actions/checkout@v3
@@ -53,7 +53,7 @@ jobs:
     runs-on: ["self-hosted"]
     strategy:
       matrix:
-        pyver: ["3.9", "3.10", "3.11", "3.12", "3.13", "3.14-rc"]
+        pyver: ["3.10", "3.11", "3.12", "3.13", "3.14"]
     steps:
       - uses: actions/checkout@v4
       - name: Build docker image
diff --git a/.github/workflows/nightly-onprem.yml b/.github/workflows/nightly-onprem.yml
index 3bcf0d26..2931ef9d 100644
--- a/.github/workflows/nightly-onprem.yml
+++ b/.github/workflows/nightly-onprem.yml
@@ -10,7 +10,7 @@ jobs:
     runs-on: ["self-hosted"]
     strategy:
       matrix:
-        pyver: ["3.9", "3.10", "3.11", "3.12", "3.13", "3.14-rc"]
+        pyver: ["3.10", "3.11", "3.12", "3.13", "3.14"]
     steps:
       - uses: actions/checkout@v4
       - name: Build docker image
diff --git a/pyproject.toml b/pyproject.toml
index 1843ffc7..6847d1e7 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -13,7 +13,7 @@ authors = [
     { name = "Allen Institute for Brain Science", email = "Marmot@AllenInstitute.onmicrosoft.com" },
 ]
 
-requires-python = ">= 3.9"
+requires-python = ">= 3.10"
 
 keywords = [
     "bioinformatics",
@@ -27,7 +27,6 @@ classifiers = [
     "License :: Other/Proprietary License",
     "Natural Language :: English",
     "Operating System :: OS Independent",
-    "Programming Language :: Python :: 3.9",
     "Programming Language :: Python :: 3.10",
     "Programming Language :: Python :: 3.11",
     "Programming Language :: Python :: 3.12",