@@ -250,25 +250,19 @@ pip install pandas numpy scipy matplotlib seaborn scikit-learn statsmodels
250250- ** apa_correlation_across_celltypes.ipynb** : Cross-cell-type APA correlation analysis
251251- ** rbp_co_occurance_dissimilarity.ipynb** : RNA-binding protein co-occurrence analysis
252252
253- #### 4. Visualization (` visualization/ ` )
254- - ** maaper_volcanos_barplots_figure6.ipynb** : Creates volcano plots and bar plots for Figure 6
255- - APA usage changes across conditions
256- - Cell-type-specific visualizations
257- - Statistical significance visualization
258-
259- #### 5. Gene Expression (` gene_expression/ ` )
253+ #### 4. Gene Expression (` gene_expression/ ` )
260254- ** DEG_ALS_genes.R** : Analysis of ALS-associated gene expression
261255- ** DEG_MAST_analysis.R** : MAST-based differential expression analysis
262256- ** DEG_pathway_analysis.R** : Pathway enrichment analysis for DEGs
263257- ** DEG_visualization.R** : Visualization of differential expression results
264258
265- #### 6 . Pathway Analysis (` pathway_analysis/ ` )
259+ #### 5 . Pathway Analysis (` pathway_analysis/ ` )
266260- ** APA_pathway_analysis.R** : Gene set enrichment analysis for APA-affected genes
267261 - GO term enrichment
268262 - Reactome pathway analysis
269263 - Custom gene set analysis
270264
271- #### 7 . Preprocessing (` preprocessing/ ` )
265+ #### 6 . Preprocessing (` preprocessing/ ` )
272266- ** processing_annotation/** : Single-cell RNA-seq processing pipeline
273267 - ` 01_snRNA_cellranger_preprocess.sh ` : Cell Ranger preprocessing
274268 - ` 02_snRNA_process_QC.R ` : Quality control and filtering
@@ -339,7 +333,7 @@ Please ensure you have access to the appropriate datasets before running the ana
339333
340334If you use this analysis pipeline, please cite our paper:
341335```
342- [Paper citation to be added upon publication]
336+ [[ Paper citation to be added upon publication]](https://www.biorxiv.org/content/10.1101/2023.12.22.573083v2)
343337```
344338
345339For questions about the analysis pipeline, please open an issue in the GitHub repository.
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