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README.md

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@@ -250,25 +250,19 @@ pip install pandas numpy scipy matplotlib seaborn scikit-learn statsmodels
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- **apa_correlation_across_celltypes.ipynb**: Cross-cell-type APA correlation analysis
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- **rbp_co_occurance_dissimilarity.ipynb**: RNA-binding protein co-occurrence analysis
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#### 4. Visualization (`visualization/`)
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- **maaper_volcanos_barplots_figure6.ipynb**: Creates volcano plots and bar plots for Figure 6
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- APA usage changes across conditions
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- Cell-type-specific visualizations
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- Statistical significance visualization
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#### 5. Gene Expression (`gene_expression/`)
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#### 4. Gene Expression (`gene_expression/`)
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- **DEG_ALS_genes.R**: Analysis of ALS-associated gene expression
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- **DEG_MAST_analysis.R**: MAST-based differential expression analysis
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- **DEG_pathway_analysis.R**: Pathway enrichment analysis for DEGs
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- **DEG_visualization.R**: Visualization of differential expression results
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#### 6. Pathway Analysis (`pathway_analysis/`)
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#### 5. Pathway Analysis (`pathway_analysis/`)
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- **APA_pathway_analysis.R**: Gene set enrichment analysis for APA-affected genes
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- GO term enrichment
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- Reactome pathway analysis
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- Custom gene set analysis
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#### 7. Preprocessing (`preprocessing/`)
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#### 6. Preprocessing (`preprocessing/`)
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- **processing_annotation/**: Single-cell RNA-seq processing pipeline
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- `01_snRNA_cellranger_preprocess.sh`: Cell Ranger preprocessing
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- `02_snRNA_process_QC.R`: Quality control and filtering
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If you use this analysis pipeline, please cite our paper:
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```
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[Paper citation to be added upon publication]
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[[Paper citation to be added upon publication]](https://www.biorxiv.org/content/10.1101/2023.12.22.573083v2)
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```
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For questions about the analysis pipeline, please open an issue in the GitHub repository.

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