Skip to content

EnrichmentMap - Cannot parse line 2 of enrichment file #557

@nmaun053

Description

@nmaun053

Hi,

I am using ClusterProfiler on R to run my GSEA and I am having trouble with the files I am exporting to EnrichmentMap on Cystoscape.

Straight out of R, the file looks like this:
ID setSize enrichmentScore NES pvalue p.adjust qvalue rank leading_edge core_enrichment core_size
GOCC_MITOCHONDRION 1781 0.423194013 2.065931975 3.59326E-46 3.57601E-42 2.69003E-42 3463 tags=34%, list=22%, signal=30% Slc11a2/App/Sdhd/Micu3/... 606

This gives me the cannot parse line 2 error when uploading as GSEA for Analysis type

And then, using the following format as reference: Supplementary_Table8_gsea_report_for_na_pos that I obtained from https://baderlab.github.io/Cytoscape_workflows/EnrichmentMapPipeline/index.html

I changed my headings to:
NAME GS SIZE ES NES pvalue qvalue p.adjust rank leading_edge
GOCC_MITOCHONDRION GOCC_MITOCHONDRION 1781 0.423194013 2.065931975 3.59326E-46 2.69003E-42 3.57601E-42 3463 tags=34%, list=22%, signal=30%

And still got the parsing error

Next, I tried using the generic option for analysis type using the second format mention, and still got the parsing error.

Then, I tried using the headings suggested for generic enrichment results from: https://enrichmentmap.readthedocs.io/en/latest/FileFormats.html, which also gave me the parsing error

ID description p-value FDR gene list
GOCC_MITOCHONDRION GOCC_MITOCHONDRION 3.57601E-42 2.69003E-42 Slc11a2,App,Sdhd,Micu3,...

For all of the above, my gmt file and rnk file stayed the same.
GMT (tab seperated):
GOBERT_CORE_OLIGODENDROCYTE_DIFFERENTIATION NA Abat Abca1

RNK:
gene score
Thy1 6.03204820036826
Slc11a2 5.92889879296482

Overall, I don't know why my enrichment file isn't working.

Thanks,
Nishi

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions