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Update all manual files
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MANUAL

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@@ -199,7 +199,7 @@ Note that [Maq] internally rounds base qualities to the nearest 10 and
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rounds qualities greater than 30 to 30. To maintain compatibility,
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Bowtie does the same. Rounding can be suppressed with the
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`--nomaqround` option.
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Bowtie is not fully sensitive in `-n` 2 and `-n` 3 modes by default.
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In these modes Bowtie imposes a "backtracking limit" to limit effort
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spent trying to find valid alignments for low-quality reads unlikely to
@@ -352,7 +352,7 @@ output no alignments.
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Specifying `-m` 5 instructs bowtie to refrain from reporting any
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alignments for reads having more than 5 reportable alignments. Since
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the read has exactly 5 reportable alignments, the `-m` 5 limit allows
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`bowtie` to print them as usual.
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`bowtie` to print them as usual.
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Example 9: `-a -m 3 --best --strata`
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@@ -383,7 +383,7 @@ A valid paired-end alignment satisfies these criteria:
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defined by the `-v`/`-n`/`-e`/`-l` options.
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2. The relative orientation and position of the mates satisfy the
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constraints defined by the `-I`/`-X`/`--fr`/`--rf`/`--ff`
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options.
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options.
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Policies governing which paired-end alignments are reported for a
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given read are specified using the `-k`, `-a` and `-m` options as
@@ -798,7 +798,7 @@ versions prior to 1.3. Default: off.
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--solexa1.3-quals
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Same as `--phred64-quals`. This is usually the right option for use
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with (unconverted) reads emitted by GA Pipeline version 1.3 or later.
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with (unconverted) reads emitted by GA Pipeline version 1.3 or later.
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Default: off.
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--integer-quals
@@ -1037,7 +1037,7 @@ disallowed via the `-k` option) and they fall into more than one
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alignment "stratum", report only those alignments that fall into the
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best stratum. By default, Bowtie reports all reportable alignments
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regardless of whether they fall into multiple strata. When
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`--strata` is specified, `--best` must also be specified.
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`--strata` is specified, `--best` must also be specified.
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Output
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@@ -1283,7 +1283,7 @@ Default `bowtie` output
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character of the alignment occurs
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5. Read sequence (reverse-complemented if orientation is `-`).
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If the read was in colorspace, then the sequence shown in this
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column is the sequence of *decoded nucleotides*, not the original
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colors. See the [Colorspace alignment] section for details about
@@ -1292,7 +1292,7 @@ Default `bowtie` output
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6. ASCII-encoded read qualities (reversed if orientation is `-`). The
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encoded quality values are on the Phred scale and the encoding is
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ASCII-offset by 33 (ASCII char `!`).
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If the read was in colorspace, then the qualities shown in this
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column are the *decoded qualities*, not the original qualities.
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See the [Colorspace alignment] section for details about decoding.
@@ -1302,7 +1302,7 @@ Default `bowtie` output
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this read, this column contains the value of the ceiling,
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indicating that at least that many valid alignments were found in
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addition to the one reported.
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Otherwise, this column contains the number of other instances where
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the same sequence aligned against the same reference characters as
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were aligned against in the reported alignment. This is *not* the
@@ -1316,7 +1316,7 @@ Default `bowtie` output
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mismatches in the alignment, this field is empty. A single
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descriptor has the format offset:reference-base>read-base. The
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offset is expressed as a 0-based offset from the high-quality (5')
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end of the read.
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end of the read.
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SAM `bowtie` output
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-------------------
@@ -1402,7 +1402,7 @@ right, the fields are:
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11. ASCII-encoded read qualities (reverse-complemented if the read
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aligned to the reverse strand). The encoded quality values are on
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the [Phred quality] scale and the encoding is ASCII-offset by 33
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(ASCII char `!`), similarly to a [FASTQ] file.
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(ASCII char `!`), similarly to a [FASTQ] file.
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12. Optional fields. Fields are tab-separated. For descriptions of
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all possible optional fields, see the SAM format specification.
@@ -1453,10 +1453,10 @@ The `bowtie-build` indexer
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`bowtie-build` outputs a set of 6 files with suffixes `.1.ebwt`,
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`.2.ebwt`, `.3.ebwt`, `.4.ebwt`, `.rev.1.ebwt`, and `.rev.2.ebwt`. (If
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the total length of all the input sequences is greater than about 4
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billion, then the index files will end in `ebwtl` instead of `ebwt`.)
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billion, then the index files will end in `ebwtl` instead of `ebwt`.)
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These files together constitute the index: they are all that is needed
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to align reads to that reference. The original sequence files are no
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longer used by Bowtie once the index is built.
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longer used by Bowtie once the index is built.
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Use of Karkkainen's [blockwise algorithm] allows `bowtie-build` to
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trade off between running time and memory usage. `bowtie-build` has
@@ -1592,7 +1592,7 @@ governs how many rows get marked: the indexer will mark every 2^`<int>`
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rows. Marking more rows makes reference-position lookups faster, but
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requires more memory to hold the annotations at runtime. The default
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is 5 (every 32nd row is marked; for human genome, annotations occupy
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about 340 megabytes).
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about 340 megabytes).
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-t/--ftabchars <int>
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