Issue with min_AHP_indices Detection

[tomko-neuron]

Hi @AurelienJaquier,

I'm using the eFEL library to extract min_AHP_values for a single action potential. According to the documentation, this feature uses min_AHP_indices. However, in my case, for some traces, the min_AHP_indices are not being found, and I can't figure out the reason why.

I am attaching two example traces:

1. An experimental trace where min_AHP_indices are found.
2. A simulation trace where min_AHP_indices are not found, despite using the same eFEL settings.

Stim_start is 5.0 ms and stim_end is 35.0 ms.

Could you please advise what might be causing this issue?

Thank you for your assistance.

Best,
Matus

Attachments: Experimental trace Simulation trace

[AurelienJaquier]

Hi @tomko-neuron
min_AHP_indices finds the first minimum after each peak, but skips if the first minimum is the last point of the trace (because it means that the 'real' minimum would realistically be after that, if we had more data points after the end of the trace, and so keeping this value would bias efel's output).
You are in this case, where for your simulation, the minimum is at the end of the trace, and is thus skipped. But for the experimental trace, we have some noise, 'artificially' creating some local minima before the end of the trace, the first of which is detected by min_AHP_indices.