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Output files

Depending on the options you choose, the following files will be created. All PSMs, peptides, and proteins are not yet filtered at any false discovery rate (FDR) level.

Main output files:

File Description
<prefix>.psms.tsv Main output file with rescored PSMs and their new scores
<prefix>.report.html HTML report with interactive plots showing the results and some quality control metrics.

Log and configuration files:

File Description
<prefix>.log.txt Log file with information about the run
<prefix>.log.html HTML version of the log file
<prefix>.full-config.json Full configuration file with all the parameters used as configured in the user-provided configuration file, the command line or graphical interface, and the default values.
<prefix>.feature_names.tsv List of the features and their descriptions
<prefix>.intermediate.psms.tsv Created automatically if the process crashes during feature generation or rescoring. Contains all PSMs with successfully added features up to the crash point. Can be used to resume processing with -p <prefix>.intermediate.psms.tsv -t tsv.

Rescoring engine files:

File Description
<prefix>.<mokapot/percolator>.psms.txt PSMs and their new scores at PSM-level FDR.
<prefix>.<mokapot/percolator>.peptides.txt Peptides and their new scores at peptide-level FDR.
<prefix>.<mokapot/percolator>.proteins.txt Proteins and their new scores at protein-level FDR.
<prefix>.<mokapot/percolator>.decoy.psms.txt Decoy PSMs and their new scores at PSM-level FDR.
<prefix>.<mokapot/percolator>.decoy.peptides.txt Decoy peptides and their new scores at peptide-level FDR.
<prefix>.<mokapot/percolator>.decoy.proteins.txt Decoy proteins and their new scores at protein-level FDR.
<prefix>.<mokapot/percolator>.weights.txt Feature weights, showing feature usage in the rescoring run

If no rescoring engine is selected, if Percolator was selected, or in DEBUG mode, the following files will also be written:

File Description
<prefix>.pin PSMs with all features for rescoring