xyzAc fitting #2
Description
Hi!
I have been using utrack3D on 3D LLS movies of endocytosis markers, and it has been working great! Thank you so much for making this amazing software freely available.
I had a quick question regarding spot detection - currently, I have been using multiscaleDetectionDebug, which outputs xCoord, yCoord, zCoord, and Amplitude. If my understanding is correct, this amplitude is not background corrected (essentially A + c). I'm growing increasingly interested in getting subvoxel detections and separating out A and c values. To do this, I tried using the point source detector in the GUI in xyzAc mode. However, the output detection file still had xCoord, yCoord, zCoord, and Amp. Could you please help me with this?
A quick side note - there is another column Int, which I find always equals the value in Amp, and every variable has two columns, the second always with a value of 0.5. Was just wondering if this is expected?
Thank you so much for your help!