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| 1 | +{ |
| 2 | + "$schema": "https://json-schema.org/draft/2020-12/schema", |
| 3 | + "$id": "https://raw.githubusercontent.com/dessimozlab/FastOMA/main/nextflow_schema.json", |
| 4 | + "title": "FastOMA Pipeline Parameters", |
| 5 | + "description": "Fast orthologous matrix analysis pipeline", |
| 6 | + "type": "object", |
| 7 | + "$defs": { |
| 8 | + "input_options": { |
| 9 | + "title": "Input options", |
| 10 | + "type": "object", |
| 11 | + "description": "Define where the pipeline should find input data ", |
| 12 | + "required": ["input_folder", "species_tree"], |
| 13 | + "properties": { |
| 14 | + "input_folder": { |
| 15 | + "type": "string", |
| 16 | + "format": "directory-path", |
| 17 | + "description": "Path to input directory containing proteomes and species tree", |
| 18 | + "fa_icon": "fas fa-folder-open" |
| 19 | + }, |
| 20 | + "proteome_folder": { |
| 21 | + "type": "string", |
| 22 | + "format": "directory-path", |
| 23 | + "description": "Path to input directory containing the proteome files in fasta format", |
| 24 | + "help": "If not provided, the proteomes are asumed to be in the input_folder/proteomes directory.", |
| 25 | + "fa_icon": "fas fa-folder-open" |
| 26 | + }, |
| 27 | + "hogmap_in": { |
| 28 | + "type": "string", |
| 29 | + "format": "directory-path", |
| 30 | + "description": "Path to input directory containing the hogmap files", |
| 31 | + "fa_icon": "fas fa-folder-open", |
| 32 | + "help": "If provided, the hogmap files will be used instead of computing them from scratch with OMAmer. This can save time if you have already computed the hogmaps for your proteomes." |
| 33 | + }, |
| 34 | + "species_tree": { |
| 35 | + "type": "string", |
| 36 | + "format": "file-path", |
| 37 | + "description": "Path to species tree file", |
| 38 | + "fa_icon": "fas fa-tree", |
| 39 | + "pattern": "^\\S+\\.(nhx|nh|nwk)", |
| 40 | + "help": "The species tree should be in Newick or NHX format. By default, the pipeline looks for a file named species_tree.nwk in the input_folder." |
| 41 | + }, |
| 42 | + "splice_folder": { |
| 43 | + "type": "string", |
| 44 | + "format": "directory-path", |
| 45 | + "description": "Path to input directory containing the splice files", |
| 46 | + "fa_icon": "fas fa-folder-open", |
| 47 | + "help": "If provided, FastOMA will use the splice files to identify and handle alternative splicing isoforms in the proteomes and select the best representative isoform for each gene." |
| 48 | + }, |
| 49 | + "omamer_db": { |
| 50 | + "type": "string", |
| 51 | + "format": "file-path", |
| 52 | + "description": "Path to OMAmer database against which the proteomes will be mapped", |
| 53 | + "fa_icon": "fas fa-database", |
| 54 | + "help": "If not provided, the default OMAmer database (LUCA) will be used.", |
| 55 | + "default": "https://omabrowser.org/All/LUCA.h5" |
| 56 | + } |
| 57 | + } |
| 58 | + }, |
| 59 | + "output_options": { |
| 60 | + "title": "Output options", |
| 61 | + "type": "object", |
| 62 | + "description": "Define where the pipeline should store the output data", |
| 63 | + "required": ["output_folder"], |
| 64 | + "properties": { |
| 65 | + "output_folder": { |
| 66 | + "type": "string", |
| 67 | + "default": "Output", |
| 68 | + "format": "directory-path", |
| 69 | + "description": "The output directory where the results will be saved", |
| 70 | + "fa_icon": "fas fa-folder-open" |
| 71 | + }, |
| 72 | + "hogmap_folder": { |
| 73 | + "type": "string", |
| 74 | + "format": "directory-path", |
| 75 | + "description": "The output directory where the omamer hogmap files will be saved", |
| 76 | + "help": "If not provided, the hogmap files will be saved in the output_folder/hogmap directory.", |
| 77 | + "fa_icon": "fas fa-folder-open", |
| 78 | + "default": "Output/hogmap" |
| 79 | + } |
| 80 | + } |
| 81 | + }, |
| 82 | + "processing_options": { |
| 83 | + "title": "Processing options", |
| 84 | + "type": "object", |
| 85 | + "description": "Parameters for processing and analysis", |
| 86 | + "properties": { |
| 87 | + "fasta_header_id_transformer": { |
| 88 | + "type": "string", |
| 89 | + "default": "noop", |
| 90 | + "enum": ["noop", "UniProt"], |
| 91 | + "description": "FASTA header ID transformer method" |
| 92 | + }, |
| 93 | + "filter_method": { |
| 94 | + "type": "string", |
| 95 | + "default": "col-row-threshold", |
| 96 | + "description": "MSA filtering method", |
| 97 | + "enum": ["col-row-threshold", "col-elbow-row-threshold", "trimal"] |
| 98 | + }, |
| 99 | + "filter_gap_ratio_row": { |
| 100 | + "type": "number", |
| 101 | + "default": 0.3, |
| 102 | + "minimum": 0, |
| 103 | + "maximum": 1, |
| 104 | + "description": "Gap ratio threshold for row filtering" |
| 105 | + }, |
| 106 | + "filter_gap_ratio_col": { |
| 107 | + "type": "number", |
| 108 | + "default": 0.5, |
| 109 | + "minimum": 0, |
| 110 | + "maximum": 1 |
| 111 | + }, |
| 112 | + "nr_repr_per_hog": { |
| 113 | + "type": "integer", |
| 114 | + "default": 5, |
| 115 | + "minimum": 1 |
| 116 | + }, |
| 117 | + "min_sequence_length": { |
| 118 | + "type": "integer", |
| 119 | + "default": 20 |
| 120 | + } |
| 121 | + } |
| 122 | + }, |
| 123 | + "output_control": { |
| 124 | + "title": "Output control", |
| 125 | + "type": "object", |
| 126 | + "description": "Control what outputs are saved", |
| 127 | + "properties": { |
| 128 | + "write_msas": { |
| 129 | + "type": "boolean", |
| 130 | + "description": "Write multiple sequence alignments" |
| 131 | + }, |
| 132 | + "write_genetrees": { |
| 133 | + "type": "boolean", |
| 134 | + "description": "Write gene trees" |
| 135 | + }, |
| 136 | + "debug_enabled": { |
| 137 | + "type": "boolean", |
| 138 | + "description": "Enable debug mode" |
| 139 | + }, |
| 140 | + "msa_folder": { |
| 141 | + "type": "string", |
| 142 | + "default": "Output/msa" |
| 143 | + }, |
| 144 | + "temp_output": { |
| 145 | + "type": "string", |
| 146 | + "default": "Output/temp_output" |
| 147 | + }, |
| 148 | + "genetrees_folder": { |
| 149 | + "type": "string", |
| 150 | + "default": "Output/genetrees" |
| 151 | + }, |
| 152 | + "force_pairwise_ortholog_generation": { |
| 153 | + "type": "boolean", |
| 154 | + "description": "Force generation of pairwise orthologs even for large datasets (may be slow)", |
| 155 | + "help": "By default, pairwise orthologs are not generated for datasets with more than 25 species to avoid long runtimes. Enable this option to override this behavior." |
| 156 | + } |
| 157 | + } |
| 158 | + }, |
| 159 | + "resource_options": { |
| 160 | + "title": "Resource options", |
| 161 | + "type": "object", |
| 162 | + "description": "", |
| 163 | + "default": "", |
| 164 | + "properties": { |
| 165 | + "time_multiplier": { |
| 166 | + "type": "integer", |
| 167 | + "default": 1 |
| 168 | + }, |
| 169 | + "memory_multiplier": { |
| 170 | + "type": "integer", |
| 171 | + "default": 1 |
| 172 | + } |
| 173 | + } |
| 174 | + }, |
| 175 | + "generic_options": { |
| 176 | + "title": "Generic options", |
| 177 | + "type": "object", |
| 178 | + "description": "Less common options for the pipeline", |
| 179 | + "properties": { |
| 180 | + "help": { |
| 181 | + "type": "boolean", |
| 182 | + "description": "Display help text", |
| 183 | + "fa_icon": "fas fa-question-circle", |
| 184 | + "hidden": true |
| 185 | + }, |
| 186 | + "report": { |
| 187 | + "type": "boolean", |
| 188 | + "description": "Generate nextflow reports" |
| 189 | + }, |
| 190 | + "statsdir": { |
| 191 | + "type": "string", |
| 192 | + "fa_icon": "fas fa-folder-open", |
| 193 | + "default": "Output/stats" |
| 194 | + }, |
| 195 | + "trace_report_suffix": { |
| 196 | + "type": "string", |
| 197 | + "fa_icon": "far calendar", |
| 198 | + "description": "Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.", |
| 199 | + "hidden": true |
| 200 | + }, |
| 201 | + "custom_config_version": { |
| 202 | + "type": "string", |
| 203 | + "default": "master" |
| 204 | + }, |
| 205 | + "custom_config_base": { |
| 206 | + "type": "string", |
| 207 | + "default": "https://raw.githubusercontent.com/nf-core/configs/master" |
| 208 | + }, |
| 209 | + "container_name": { |
| 210 | + "type": "string", |
| 211 | + "default": "dessimozlab/fastoma" |
| 212 | + }, |
| 213 | + "container_version": { |
| 214 | + "type": "string", |
| 215 | + "default": "dev" |
| 216 | + } |
| 217 | + } |
| 218 | + } |
| 219 | + }, |
| 220 | + "allOf": [ |
| 221 | + { |
| 222 | + "$ref": "#/$defs/input_options" |
| 223 | + }, |
| 224 | + { |
| 225 | + "$ref": "#/$defs/output_options" |
| 226 | + }, |
| 227 | + { |
| 228 | + "$ref": "#/$defs/processing_options" |
| 229 | + }, |
| 230 | + { |
| 231 | + "$ref": "#/$defs/output_control" |
| 232 | + }, |
| 233 | + { |
| 234 | + "$ref": "#/$defs/resource_options" |
| 235 | + }, |
| 236 | + { |
| 237 | + "$ref": "#/$defs/generic_options" |
| 238 | + } |
| 239 | + ] |
| 240 | +} |
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