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***[CellProfiler command line flags](https://github.com/CellProfiler/CellProfiler/wiki/Adapting-CellProfiler-to-a-LIMS-environment#cmd):** These can be modified in the cp-worker script.
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***Log stream names or logging level:** These can be modified in the cp-worker script.
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## Changes to CellProfiler pipeline to use Distributed-CellProfiler with Runcellpose plugin
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## Changes to CellProfiler pipeline to use Distributed-CellProfiler with RunCellpose plugin
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***Distributed-CellProfiler version:** At least CellProfiler version 4.2.4, and use the DOCKERHUB_TAG on config.py as `bethcimini/distributed-cellprofiler:2.1.0_4.2.4_plugins`.
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***Custom model:** the user could be interested in using a [User-trained model](https://cellpose.readthedocs.io/en/latest/models.html).
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***AWS:** If using custom model generated using cellpose, add the model file to the folder in the following structure:
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***Distributed-CellProfiler version:** At least CellProfiler version 4.2.4, and use the DOCKERHUB_TAG in config.py as `bethcimini/distributed-cellprofiler:2.1.0_4.2.4_plugins`.
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***Custom model: If using a [custom User-trained model](https://cellpose.readthedocs.io/en/latest/models.html) generated using Cellpose, add the model file to S3.
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We use the following structure to organize our files on S3.
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```
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├── <project_name>
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├── workspace
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│ ├── model
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││├── custom_model_filename
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└── <project_name>
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└── workspace
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└── model
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└── custom_model_filename
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```
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***RunCellpose module:**
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* Inside RunCellpose you should select the Detection mode to "custom". Then, it will ask you for "Location of the pre-trained model file". There paste the path (adapted to your project): **/home/ubuntu/bucket/projects/<project_name>/workspace/model/**
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* Paste the custom_model_filename to "Pre-trained model file name" field.
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* Inside RunCellpose, select the "custom" Detection mode.
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In "Location of the pre-trained model file", enter the mounted bucket path to your model.
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e.g. **/home/ubuntu/bucket/projects/<project_name>/workspace/model/**
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* In "Pre-trained model file name", enter your custom_model_filename
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