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examples/M16/README.md

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@@ -10,7 +10,7 @@ And the performance can be evaluated from multiple aspects, such as the predicti
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The data preprocessing methods can generate data partitions to enable flexible cross-validation analysis, normalize and remove batch effects from gene expression data of cancer cells, and generate genomic representations at the gene set level for cancer cells.
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The feature selection methods can filter features based on missing values and variations, and perform feature decorrelation.
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Features without much variation might not be useful for prediction and highly-correlated features are not necessary to be all included in the prediction model.
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We also implement and extend the co-expression extrapolation (COXEN) gene selection method for Pilot 1 project, which can select predictive and generalizable genes for predicting drug response in the precision oncology applications.
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We also implement and extend the co-expression extrapolation (COXEN) gene selection method for Pilot 1 project [3], which can select predictive and generalizable genes for predicting drug response in the precision oncology applications.
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## General Data Preprocessing Functions
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```quantile_normalizationa```
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To perform quantile normalization of genomic data [8] with tolerance of missing values.
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To perform quantile normalization of genomic data [1] with tolerance of missing values.
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```combat_batch_effect_removal```
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To perform ComBat analysis [9] on gene expression data to remove batch effects.
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To perform ComBat analysis [2] on gene expression data to remove batch effects.
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```generate_gene_set_data```
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```coxen_single_drug_gene_selection```
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To perform co-expression extrapolation (COXEN) analysis that selects predictive and generalizable genes for predicting the response of tumor cells to a specific drug.
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To perform co-expression extrapolation (COXEN) analysis [3] that selects predictive and generalizable genes for predicting the response of tumor cells to a specific drug.
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```coxen_multi_drug_gene_selection```
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Average median of CCLE cell lines is 2.72
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Average first quartile of CCLE cell lines is 0.13
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```
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# References
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1. Bolstad BM, Irizarry RA, Astrand M, et al. \(2003\) A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics. 2003 Jan 22;19\(2\):185-93.
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2. Johnson WE, Rabinovic A, and Li C \(2007\) Adjusting batch effects in microarray expression data using Empirical Bayes methods. Biostatistics 8\(1\):118-127.
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3. Lee JK, Havaleshko DM, Cho H, et al. \(2007\) A strategy for predicting the chemosensitivity of human cancers and its application to drug discovery. Proc Natl Acad Sci USA, 2007 Aug 7; 104\(32\):13086-91. Epub 2007 Jul 31
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