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Bump dada2 (galaxyproject#6731)
* add comment on pooled chimera removal * use standard macro token names and add profile token * bump * workaround link
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tools/dada2/dada2_assignTaxonomyAddspecies.xml

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<tool id="dada2_assignTaxonomyAddspecies" name="dada2: assignTaxonomy and addSpecies" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_assignTaxonomyAddspecies" name="dada2: assignTaxonomy and addSpecies" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>Learn Error rates</description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_dada.xml

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<tool id="dada2_dada" name="dada2: dada" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_dada" name="dada2: dada" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>Remove sequencing errors</description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_filterAndTrim.xml

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<tool id="dada2_filterAndTrim" name="dada2: filterAndTrim" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_filterAndTrim" name="dada2: filterAndTrim" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>Filter and trim short read data</description>
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<macros>
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<import>macros.xml</import>
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- Truncation of the read length is enforced after trimming of the right end.
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- The long read filter is applied before trimming and the short read filter after trimming.
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- For details on the calculation of the number of expected errors see also https://doi.org/10.1093/bioinformatics/btv401
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- For details on the calculation of the number of expected errors see also Calahan et al. 2016
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*String present at the start of valid reads* (orient.fwd):
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@@ -316,4 +315,4 @@ This step may be replaced by alternative tools to filter and trim short read dat
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@HELP_OVERVIEW@
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]]></help>
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<expand macro="citations"/>
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</tool>
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</tool>

tools/dada2/dada2_learnErrors.xml

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<tool id="dada2_learnErrors" name="dada2: learnErrors" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_learnErrors" name="dada2: learnErrors" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>Learn Error rates</description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_makeSequenceTable.xml

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<tool id="dada2_makeSequenceTable" name="dada2: makeSequenceTable" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_makeSequenceTable" name="dada2: makeSequenceTable" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>construct a sequence table (analogous to OTU table)</description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_mergePairs.xml

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<tool id="dada2_mergePairs" name="dada2: mergePairs" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_mergePairs" name="dada2: mergePairs" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>Merge denoised forward and reverse reads</description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_plotComplexity.xml

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<tool id="dada2_plotComplexity" name="dada2: plotComplexity" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_plotComplexity" name="dada2: plotComplexity" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>Plot sequence complexity profile</description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_plotQualityProfile.xml

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<tool id="dada2_plotQualityProfile" name="dada2: plotQualityProfile" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_plotQualityProfile" name="dada2: plotQualityProfile" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>plot a visual summary of the quality scores</description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_primercheck.xml

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<tool id="dada2_primerCheck" name="dada2: primer check" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_primerCheck" name="dada2: primer check" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description></description>
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<macros>
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<import>macros.xml</import>

tools/dada2/dada2_removeBimeraDenovo.xml

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<tool id="dada2_removeBimeraDenovo" name="dada2: removeBimeraDenovo" version="@DADA2_VERSION@+galaxy@WRAPPER_VERSION@" profile="19.09">
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<tool id="dada2_removeBimeraDenovo" name="dada2: removeBimeraDenovo" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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<description>Remove bimeras from collections of unique sequences</description>
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<macros>
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<import>macros.xml</import>
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- from **pooled** sequences: Each sequence is evaluated against a set of "parents" drawn from the sequence collection that are sufficiently more abundant than the sequence being evaluated. Sequences that are bimera are removed, i.e. a two-parent chimera, in which the left side is made up of one parent sequence, and the right-side made up of a second parent sequence.
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- by **consensus**: In short, bimeric sequences are flagged on a sample-by-sample basis. Then, a vote is performed for each sequence across all samples in which it appeared. If the sequence is flagged in a sufficiently high fraction of samples, it is identified as a bimera. A logical vector is returned, with an entry for each sequence in the table indicating whether it was identified as bimeric by this consensus procedure.
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**Note**: pooled should only be used in combination with pooled denoising.
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Usage
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