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Different result compared to other software #8
Description
Dear developers,
I'm calculating the double enzyme restriction sites on the Asparagous officinalis genome and i'm comparing the results with other software. As you can see from the table below, in some cases the number of predicted loci is consisent between the two software, while in other it is not (e.g. MseI, TaqaI).
| Enzyme1 | Enzyme2 | GBSX | RAPYDS |
|---|---|---|---|
| ApeKI | BfaI | 61.742 | 61.356 |
| EcoRI | ApeKI | 11.313 | 37.965 |
| EcoRI | MspI | 7.750 | 7.434 |
| MseI | ApeKI | 62.211 | 60.408 |
| MseI | TaqaI | 83.264 | 303.430 |
| MspI | BfaI | 42.005 | 146.290 |
| PstI | ApeKI | 6.081 | 10.181 |
| PstI | TaqaI | 8.908 | 8.688 |
I belive this is something related to a different approach, or different filters that the two alghoritms have, and for sure i can't say which is right. Do you have any clue from what it can be due this discrepanciy? Maybe it is related to the enzyme target sequence? Or from different cutting efficiency calculation?
Thanks in advance,
Luca