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Description
Hello,
I'm running bambu for the first time following the "General usage" example, and getting the warnings below. R version 4.5.1 had some issues, so I have used R4.5.2; you indicated you used a patched version of 4.5.1.
Your help in resolving the warnings will be highly appreciated, thank you very much!
Roumi
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(1) [16:34:04] WARNING: src/learner.cc:553:
If you are loading a serialized model (like pickle in Python, RDS in R) generated by
older XGBoost, please export the model by calling Booster.save_model from that version
first, then load it back in current version. See:
https://xgboost.readthedocs.io/en/latest/tutorials/saving_model.html
for more details about differences between saving model and serializing.
#5 copies of this warning
(3) Warning message:
Returning more (or less) than 1 row per summarise() group was deprecated in dplyr 1.1.0.
ℹ Please use reframe() instead.
ℹ When switching from summarise() to reframe(), remember that reframe() always returns an ungrouped data
frame and adjust accordingly.
ℹ The deprecated feature was likely used in the bambu package.
Please report the issue to the authors.
This warning is displayed once every 8 hours.
Call lifecycle::last_lifecycle_warnings() to see where this warning was generated.
(2) - probably dependent on the example data:
Detected 3 warnings across the samples during read class construction. Access warnings with metadata(bambuOutput)$warnings
--- Start extending annotations ---
WARNING - Less than 50 TRUE or FALSE read classes for NDR precision stabilization.
NDR will be approximated as: (1 - Transcript Model Prediction Score)
Using a novel discovery rate (NDR) of: 0
WARNING - No novel transcripts meet the given thresholds. Try a higher NDR.
--- Start isoform quantification ---
--- Finished running Bambu ---
This is my sessionInfo():
R version 4.5.2 (2025-10-31)
Platform: x86_64-pc-linux-gnu
Running under: AlmaLinux 8.8 (Sapphire Caracal)
Matrix products: default
BLAS: /hpf/tools/alma8/R/4.5.2/lib64/R/lib/libRblas.so
LAPACK: /hpf/tools/alma8/R/4.5.2/lib64/R/lib/libRlapack.so; LAPACK version 3.12.1
locale:
[1] LC_CTYPE=en_CA.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_CA.UTF-8 LC_COLLATE=en_CA.UTF-8
[5] LC_MONETARY=en_CA.UTF-8 LC_MESSAGES=en_CA.UTF-8
[7] LC_PAPER=en_CA.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_CA.UTF-8 LC_IDENTIFICATION=C
time zone: America/Toronto
tzcode source: system (glibc)
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods
[8] base
other attached packages:
[1] NanoporeRNASeq_1.20.0 ExperimentHub_3.0.0
[3] AnnotationHub_4.0.0 BiocFileCache_3.0.0
[5] dbplyr_2.5.1 BiocVersion_3.22.0
[7] BiocManager_1.30.27 bambu_3.12.0
[9] BSgenome_1.78.0 rtracklayer_1.70.0
[11] BiocIO_1.20.0 Biostrings_2.78.0
[13] XVector_0.50.0 SummarizedExperiment_1.40.0
[15] Biobase_2.70.0 GenomicRanges_1.62.0
[17] Seqinfo_1.0.0 IRanges_2.44.0
[19] S4Vectors_0.48.0 BiocGenerics_0.56.0
[21] generics_0.1.4 MatrixGenerics_1.22.0
[23] matrixStats_1.5.0
loaded via a namespace (and not attached):
[1] KEGGREST_1.50.0 httr2_1.2.1 rjson_0.2.23
[4] lattice_0.22-7 vctrs_0.6.5 tools_4.5.2
[7] bitops_1.0-9 curl_7.0.0 parallel_4.5.2
[10] tibble_3.3.0 AnnotationDbi_1.72.0 RSQLite_2.4.4
[13] blob_1.2.4 pkgconfig_2.0.3 Matrix_1.7-4
[16] data.table_1.17.8 cigarillo_1.0.0 lifecycle_1.0.4
[19] compiler_4.5.2 Rsamtools_2.26.0 codetools_0.2-20
[22] GenomeInfoDb_1.46.0 RCurl_1.98-1.17 yaml_2.3.10
[25] pillar_1.11.1 crayon_1.5.3 tidyr_1.3.1
[28] BiocParallel_1.44.0 DelayedArray_0.36.0 cachem_1.1.0
[31] abind_1.4-8 tidyselect_1.2.1 purrr_1.2.0
[34] dplyr_1.1.4 restfulr_0.0.16 fastmap_1.2.0
[37] grid_4.5.2 cli_3.6.5 SparseArray_1.10.2
[40] magrittr_2.0.4 S4Arrays_1.10.0 GenomicFeatures_1.62.0
[43] XML_3.99-0.20 withr_3.0.2 filelock_1.0.3
[46] rappdirs_0.3.3 UCSC.utils_1.6.0 bit64_4.6.0-1
[49] xgboost_1.7.11.1 httr_1.4.7 bit_4.6.0
[52] png_0.1-8 memoise_2.0.1 rlang_1.1.6
[55] Rcpp_1.1.0 glue_1.8.0 DBI_1.2.3
[58] jsonlite_2.0.0 R6_2.6.1 GenomicAlignments_1.46.0