ReadsInPromoter larger than nFrags

[pchiang5]

Hello,

I got the following output from my ArchR project:

adata.obs.ReadsInTSS.mean()

4759.797468354431

adata.obs.nFrags.mean()

20011.732067510547

adata.obs.PromoterRatio.mean()

1.043950311252914

adata.obs.ReadsInPromoter.mean()

34353.31012658228

My understanding is that the ReadInPromoter shall always smaller than the total fragment numbers (nFrags), as ReadsInTSS be. However, the mean of ReadInPromoter was larger than nFrags in this particular dataset and PromoterRatio of some cells are even 2.35 . Is there any cause for this? Thank you.

[rcorces]

Mean probably isnt the right metric to be using here. For any given cell, the number of ReadsInPromoter cannot be greater than 2 * nFrags

Also, fragments are different than reads. 1 fragment = 2 reads.