Error with addGeneIntegrationMatrix #2137
Unanswered
Bioanya
asked this question in
Questions / Documentation
Replies: 0 comments
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
Uh oh!
There was an error while loading. Please reload this page.
-
Hello,
I am trying to integrate RNA with my ATAC data, the code I used given here and also attached the log file, please suggest me what is wrong / missing.
ArchR-addGeneIntegrationMatrix-14889e2e732de5-Date-2024-03-14_Time-12-35-16.log
proj <- addGeneIntegrationMatrix(
ArchRProj = proj,
useMatrix = "GeneScoreMatrix",
matrixName = "GeneIntegrationMatrix",
reducedDims = "IterativeLSI",
seRNA = seu_MEA,
addToArrow = TRUE,
groupRNA = "FinalCell", #metadata name in scrna seurat object used for prediction
nameCell = "predictedCell", #colnames given for archR obj
nameGroup = "predictedGroup", #colnames given for archR obj
nameScore = "predictedScore", #colnames given for archR obj
force = TRUE
)
ArchR logging to : ArchRLogs/ArchR-addGeneIntegrationMatrix-14889e2e732de5-Date-2024-03-14_Time-12-35-16.log
If there is an issue, please report to github with logFile!
2024-03-14 12:35:17 : Running Seurat's Integration Stuart* et al 2019, 0.007 mins elapsed.
2024-03-14 12:35:17 : Checking ATAC Input, 0.008 mins elapsed.
2024-03-14 12:35:21 : Checking RNA Input, 0.079 mins elapsed.
2024-03-14 12:35:28 : Found 18438 overlapping gene names from gene scores and rna matrix!, 0.196 mins elapsed.
2024-03-14 12:35:28 : Creating Integration Blocks, 0.196 mins elapsed.
2024-03-14 12:35:30 : Prepping Interation Data, 0.23 mins elapsed.
2024-03-14 12:35:31 : Computing Integration in 4 Integration Blocks!, 0 mins elapsed.
2024-03-14 12:35:31 : Block (1 of 4) : Computing Integration, 0.001 mins elapsed.
2024-03-14 12:35:31 : Block (2 of 4) : Computing Integration, 0.001 mins elapsed.
2024-03-14 12:35:31 : Block (3 of 4) : Computing Integration, 0.001 mins elapsed.
2024-03-14 12:35:31 : Block (4 of 4) : Computing Integration, 0.002 mins elapsed.
2024-03-14 12:35:36 : Block (3 of 4) : Identifying Variable Genes, 0.075 mins elapsed.
2024-03-14 12:35:36 : Block (2 of 4) : Identifying Variable Genes, 0.076 mins elapsed.
2024-03-14 12:35:36 : Block (4 of 4) : Identifying Variable Genes, 0.076 mins elapsed.
2024-03-14 12:35:36 : Block (1 of 4) : Identifying Variable Genes, 0.077 mins elapsed.
2024-03-14 12:35:38 : Block (3 of 4) : Getting GeneScoreMatrix, 0.112 mins elapsed.
2024-03-14 12:35:38 : Block (4 of 4) : Getting GeneScoreMatrix, 0.114 mins elapsed.
2024-03-14 12:35:38 : Block (2 of 4) : Getting GeneScoreMatrix, 0.115 mins elapsed.
2024-03-14 12:35:38 : Block (1 of 4) : Getting GeneScoreMatrix, 0.116 mins elapsed.
2024-03-14 12:36:24 : Block (2 of 4) : Imputing GeneScoreMatrix, 0.883 mins elapsed.
2024-03-14 12:36:24 : Block (4 of 4) : Imputing GeneScoreMatrix, 0.884 mins elapsed.
2024-03-14 12:36:24 : Block (1 of 4) : Imputing GeneScoreMatrix, 0.886 mins elapsed.
2024-03-14 12:36:25 : Block (3 of 4) : Imputing GeneScoreMatrix, 0.888 mins elapsed.
Getting ImputeWeights
Getting ImputeWeights
Getting ImputeWeights
2024-03-14 12:36:59 : Block (4 of 4) : Seurat FindTransferAnchors, 1.466 mins elapsed.
Getting ImputeWeights
2024-03-14 12:37:00 : Block (2 of 4) : Seurat FindTransferAnchors, 1.473 mins elapsed.
2024-03-14 12:37:00 : Block (1 of 4) : Seurat FindTransferAnchors, 1.477 mins elapsed.
2024-03-14 12:37:02 : Block (3 of 4) : Seurat FindTransferAnchors, 1.516 mins elapsed.
<simpleError: The new data doesn't have the same number of cells as the current data>
<simpleError: The new data doesn't have the same number of cells as the current data>
<simpleError: The new data doesn't have the same number of cells as the current data>
<simpleError: The new data doesn't have the same number of cells as the current data>
Error in .safelapply(seq_along(blockList), function(i) { :
Error Found Iteration 1 :
[1] "Error in .retryCatch({ : \n"
<simpleError in .retryCatch({ gc() Seurat::FindTransferAnchors(reference = subRNA, query = seuratATAC, reduction = reduction, features = genesUse, verbose = FALSE, ...)}, maxAttempts = 2, logFile = logFile): >
Error Found Iteration 2 :
[1] "Error in .retryCatch({ : \n"
<simpleError in .retryCatch({ gc() Seurat::FindTransferAnchors(reference = subRNA, query = seuratATAC, reduction = reduction, features = genesUse, verbose = FALSE, ...)}, maxAttempts = 2, logFile = logFile): >
Error Found Iteration 3 :
[1] "Error in .retryCatch({ : \n"
<simpleError in .retryCatch({ gc() Seurat::FindTransferAnchors(reference = subRNA, query = seuratATAC, reduction = reduction, features = genesUse, verbose = FALSE, ...)}, maxAttempts = 2, logFile = logFile): >
Error Found Iteration 4 :
[1] "Error in .retryCatch({ : \n"
<simpleError in .retryCatch({ gc() S
In addition: Warning message:
In mclapply(..., mc.cores = threads, mc.preschedule = preschedule) :
4 function calls resulted in an error
Beta Was this translation helpful? Give feedback.
All reactions