Monocle trajevtory is not running in my hand #2299
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A-legac45
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Hello,
I have this issue when trying to use getMonocleTrajectories in parallele I alrready run monocle3 on my dataset and it works. How can I also precise to do it on genescore and not geneexpressionmatrix? is it by using umap-atac and not umap_rna?
cds <- getMonocleTrajectories( ArchRProj = proj_subset,
name = "M_monocle_pseudo", useGroups = c("C1", "C2","C3", "C4", "C5"), principalGroup = "C1", groupBy = "new_cluster_namesatac_janv", embedding = "UMAP_ATAC",graphParams = list(use_partition = F, close_loop = F, learn_graph_control = list(minimal_branch_len = 25, ncenter = 1000)), clusterParams = list(k = 10))
ERROR
Running Monocole3 Trajectory Infrastructure!
Adding Embedding
Clustering Embedding
Learning Graphs
Getting Principal Node
Ordering Cells
Plotting Results - /Monocole3/Plot-Results-M_monocle_pseudo.pdf
Error in g$grobs[[legend]] : no such index at level 2
From monocle 3 (no cluster supervised) and archr trajectory which I perform previously by isolating the cells identified by monocle 3 such as
idxPassM <- which(proj_mat$cells %in%cds_pseudo_indicesM)
cellsPassM <- proj_mat$cellNames[idxPassM]
ARCHR_M_MONOCLE1_NEW <- proj_mat[cellsPassM, ]
and then implemented
ARCHR_M_MONOCLE1 <- addTrajectory(
ArchRProj = proj_mat,
name = "M_monocle_1preFilterQuantile_avril",
groupBy = "MONOCLEtraj_mars",
trajectory = trajectory_monocle_order,
embedding = "UMAP_ATAC",
preFilterQuantile = 1,## Paramètre pour afficher toutes les cellules dans pseudotimes
postFilterQuantile = 1,## Paramètre pour afficher toutes les cellules dans pseudotimes
reducedDims = "LSI_ATAC",
force = TRUE)
I observe small differences this is why I wanted to try your new version;
I have two trajectory with the same origin which I would like to implement in the same archr project is that possible?
Many thanks
Anne-laure
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