how to add motif sequence in plotFootprints #975
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lilwo
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Questions / Documentation
Replies: 2 comments
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Those motif PWM images were added manually using Adobe Illustrator. We do not provide code to generate images of motif PWMs. |
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Thanks a lot
…---Original---
From: "Ryan ***@***.***>
Date: Fri, Aug 13, 2021 23:18 PM
To: ***@***.***>;
Cc: ***@***.******@***.***>;
Subject: Re: [GreenleafLab/ArchR] how to add motif sequence in plotFootprints (#975)
Those motif PWM images were added manually using Adobe Illustrator. We do not provide code to generate images of motif PWMs.
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In the paper "ArchR is a scalable software package for integrative single-cell chromatin accessibility analysis"
fig4i–k, have motif sequence in the topleft
I found the code "ArchR_PostAnalysis_Large_Heme_Analysis.R
seFoot_None <- plotFootprints(
ArchRProj = proj,
positions = motifPositions[stringr::str_split(colnames(p),pattern=" ", simplify=TRUE)[,1]],
groupBy = "ClustFinal",
normMethod = "none",
plotName = "Footprints-No-Normalization"
)
seFoot_None2 <- plotFootprints(
ArchRProj = proj,
inputSE = seFoot_None,
positions = motifPositions[stringr::str_split(colnames(p),pattern=" ", simplify=TRUE)[,1]],
groupBy = "ClustFinal",
normMethod = "none",
plotName = "Footprints-No-Normalization",
smoothWindow = NULL
)
but the new ArchR ,plotFootprints doesn't have the inputSE, and I can't find any parameter about adding motif sequence
so is there any way to add motif sequence?
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