@@ -57,7 +57,7 @@ If you don't have Napari's 'Cells (3D + 2Ch)' image open, then open it with:
5757
5858Make sure you only have 'nuclei' in the layer list. Select any additional
5959layers, then click the ![ ] (
60- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/delete.svg
60+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/delete.svg
6161){alt="A screenshot of Napari's delete layer button" height='30px'} icon to
6262remove them. Also, select the nuclei layer (should be highlighted in blue), and
6363change its colormap from 'green' to 'gray' in the layer controls.
@@ -130,7 +130,7 @@ You should see a mask appear that highlights the nuclei in brown. If we set the
130130nuclei contrast limits back to normal (select 'nuclei' in the layer list, then
131131drag the left contrast limits node back to zero), then toggle on/off the mask
132132or nuclei layers with the ![ ] (
133- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/visibility.svg
133+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/visibility.svg
134134){alt="A screenshot of Napari's eye button" height='30px'} icon, you should see
135135that the brown areas match the nucleus boundaries reasonably well. They aren't
136136perfect though! The brown regions have a speckled appearance where some regions
@@ -303,7 +303,7 @@ Let's open the cells image in Napari again:
303303
304304As before, make sure you only have 'nuclei' in the layer list. Select any
305305additional layers, then click the ![ ] (
306- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/delete.svg
306+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/delete.svg
307307){alt="A screenshot of Napari's delete layer button" height='30px'} icon to
308308remove them. Also, select the nuclei layer (should be highlighted in blue), and
309309change its colormap from 'green' to 'gray' in the layer controls.
@@ -527,11 +527,11 @@ shrink in size. Increasing the 'radius' of the filter enhances the effect.
527527First, let's clean up our layer list. Make sure you only have the 'nuclei' and
528528'Result of gaussian_blur' layers in the layer list - select any others and
529529remove them by clicking the ![ ] (
530- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/delete.svg
530+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/delete.svg
531531){alt="A screenshot of Napari's delete layer button" height='30px'} icon. Also,
532532close all filter settings panels on the right side of Napari (apart from the
533533gaussian settings) by clicking the tiny ![ ] (
534- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/visibility_off.svg
534+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/visibility_off.svg
535535){alt="A screenshot of Napari's hide button" height='20px'} icon at their top
536536left corner.
537537
@@ -585,11 +585,11 @@ processing, and it's good to keep an eye out for when this is required!
585585
586586Let's return to thresholding our image. Close the gaussian panel by clicking the
587587tiny ![ ] (
588- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/visibility_off.svg
588+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/visibility_off.svg
589589){alt="A screenshot of Napari's hide button" height='20px'} icon at its top left
590590corner. Then select the 'blurred_mask' in the layer list and remove it by
591591clicking the ![ ] (
592- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/delete.svg
592+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/delete.svg
593593){alt="A screenshot of Napari's delete layer button" height='30px'} icon.
594594Finally, open the ` napari-matplotlib ` histogram again with:
595595` Plugins > napari Matplotlib > Histogram `
@@ -655,7 +655,7 @@ clear that some areas are still missed or incorrectly labelled.
655655First, let's clean up our layer list again. Make sure you only have the
656656'nuclei', 'mask', 'blurred_mask' and 'Result of gaussian_blur' layers in the
657657layer list - select any others and remove them by clicking the ![ ] (
658- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/delete.svg
658+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/delete.svg
659659){alt="A screenshot of Napari's delete layer button" height='30px'} icon. Then,
660660if you still have the ` napari-matplotlib ` histogram open, close it by clicking
661661the tiny ` x ` icon in the top left corner.
@@ -682,10 +682,10 @@ name in the layer list - for example, rename 'mask' to 'manual_mask',
682682'blurred_mask' to 'manual_blurred_mask', and 'Result of threshold_otsu' to
683683'otsu_blurred_mask'. Recall that you can change the colour of a mask by clicking
684684the ![ ] (
685- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/shuffle.svg
685+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/shuffle.svg
686686){alt="A screenshot of Napari's shuffle button" height='30px'} icon in the top
687687row of the layer controls. By toggling on/off the relevant ![ ] (
688- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/visibility.svg
688+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/visibility.svg
689689){alt="A screenshot of Napari's eye button" height='30px'} icons, you should see
690690that Otsu chooses a slightly different threshold than we did in our
691691'manual_blurred_mask', labelling slightly smaller regions as nuclei in the final
@@ -727,7 +727,7 @@ How do they compare to standard Otsu thresholding?
727727` Tools > Segmentation / binarization > Threshold (Otsu et al 1979, scikit-image, nsbatwm) `
728728
729729Recall that you can change the colour of a mask by clicking the ![ ] (
730- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/shuffle.svg
730+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/shuffle.svg
731731){alt="A screenshot of Napari's shuffle button" height='30px'} icon in the top
732732row of the layer controls.
733733
@@ -787,10 +787,10 @@ the 'Napari assistant'.
787787
788788First, let's clean up our layer list again. Make sure you only have the 'nuclei'
789789layer in the layer list - select any others and remove them by clicking the ![ ] (
790- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/delete.svg
790+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/delete.svg
791791){alt="A screenshot of Napari's delete layer button" height='30px'} icon. Also,
792792close all settings panels on the right side of Napari by clicking the tiny ![ ] (
793- https://raw.githubusercontent.com/napari/napari/main/napari/resources/icons/visibility_off.svg
793+ https://raw.githubusercontent.com/napari/napari/main/src/ napari/resources/icons/visibility_off.svg
794794){alt="A screenshot of Napari's hide button" height='20px'} icon at their top
795795left corner.
796796
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