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Merge remote-tracking branch 'origin/main' into repo-reorg
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scripts/Figure6and10.R

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@@ -8,13 +8,13 @@ library(ggpubr) # For publication-ready themes in ggplot2
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#------------------------------------------------------------
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# Set working directory to the path where the project files are stored
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setwd("/fs/ess/PAS2136/Hawaii-2025/beetles_intake/")
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setwd("/user/beetles_intake") #path anonymized
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# Define NEON data product ID for beetle data
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Beetle_dpID <- "DP1.10022.001"
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# Load NEON API token for accessing the data
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NEON_TOKEN <- read.delim("/users/PAS2136/alyeast/Beetle/QC/NEON_Token_AE", header = FALSE)[1, 1] # Read in the NEON API token
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NEON_TOKEN <- read.delim("/user/Token", header = FALSE)[1, 1] # Read in the NEON API token path anonymized
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# Load the beetle data from NEON API using the 'neonUtilities' package
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NEON_df <- neonUtilities::loadByProduct(
@@ -123,7 +123,7 @@ unique_beetle$scientificName <- factor(unique_beetle$scientificName,
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levels = names(sort(table(unique_beetle$scientificName), decreasing = FALSE)))
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# Create a bar plot for species abundance in the PUUM dataset
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png("/users/PAS2136/alyeast/Beetle/DataVis/BeetlePUUM_abundance.png", width = 10, height = 5, units = "in", res = 300) # Set output file and resolution
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png("./BeetlePUUM_abundance.png", width = 10, height = 5, units = "in", res = 300) # Set output file and resolution
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ggplot(data = unique_beetle, aes(x = scientificName)) + # Create bar plot for scientificName (species)
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geom_bar() + # Add bars for the count of each species
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geom_text(stat='count', aes(label = ..count..), hjust = -0.3, size = 3, angle = 90) +# Add count labels above the bars
@@ -158,7 +158,7 @@ total_counts <- combined_data_merge %>%
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group_by(scientificName.x) %>%
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summarise(total = n(), .groups = "drop")
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png("/users/PAS2136/alyeast/Beetle/DataVis/BeetlePUUM_abundance.png", width = 6.5, height = 4, units = "in", res = 300) # Set output file and resolution
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png("./BeetlePUUM_abundance.png", width = 6.5, height = 4, units = "in", res = 300) # Set output file and resolution
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ggplot(data = combined_data_merge, aes(x = scientificName.x, fill = imaged)) +
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geom_bar() +
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geom_text(data = total_counts, aes(x = scientificName.x, y = total, label = total),
@@ -215,7 +215,7 @@ dim(table(meta_Plooza$genus_spp))/dim(table(all$scientificName))
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dim(table(meta_Plooza$NEON_sampleID))/dim(table(all$sampleID))
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# Create a bar plot for species abundance in the BeetlePalooza dataset
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png("/users/PAS2136/alyeast/Beetle/DataVis/BeetlePalooza_abundance.png", width = 10, height = 10, units = "in", res = 300)# Set output file and resolution
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png("./BeetlePalooza_abundance.png", width = 10, height = 10, units = "in", res = 300)# Set output file and resolution
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ggplot(data = meta_Plooza, aes(x = genus_spp)) + # Create bar plot for genus_species (combined genus and species)
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geom_bar(fill="#ba1419") + # Add bars for the count of each genus_species combination
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geom_text(stat='count', aes(label = ..count..), hjust = -0.3, size = 3) + # Add count labels above the bars
@@ -239,7 +239,7 @@ all$spp_imaged <- ifelse(
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# (optional) turn into a factor with a consistent ordering
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all$spp_imaged <- factor(all$spp_imaged, levels = c("unimaged","imaged"))
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png("/users/PAS2136/alyeast/Beetle/DataVis/BeetlePalooza_NEON_abundance.png", width = 5, height = 4, units = "in", res = 300)# Set output file and resolution
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png("./BeetlePalooza_NEON_abundance.png", width = 5, height = 4, units = "in", res = 300)# Set output file and resolution
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ggplot(data = all, aes(x = scientificName, fill=spp_imaged)) + # Create bar plot for genus_species (combined genus and species)
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geom_bar() + # Add bars for the count of each genus_species combination
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# geom_text(stat='count', aes(label = ..count..), hjust = -0.3, size = 3) + # Add count labels above the bars
@@ -270,7 +270,7 @@ img_spp_key<-as.data.frame(img_spp_key[,c("ImageFileName","Species")])
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img_spp_key$file<-paste0("IMG_",substr(img_spp_key$ImageFileName, 5,nchar(img_spp_key$ImageFileName)),".JPG")
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#Read in file with body sizes from annotations
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body_size<-read.csv("/fs/ess/PAS2136/Hawaii-2025/beetles_intake/BeetlePUUM/Annotations/traits_metadata.csv")
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body_size<-read.csv("./BeetlePUUM/Annotations/traits_metadata.csv")
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head(body_size)
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head(img_spp_key)
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body_size$toras_path<-paste0(substr(body_size$ImageFilePath, 14, (nchar(body_size$ImageFilePath)-4)),".JPG")
@@ -284,7 +284,7 @@ species_counts <- table(body_size$Species)
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legend_labels <- paste0(names(species_counts), " (n=", species_counts, ")")
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names(legend_labels) <- names(species_counts) # Assign species as names
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png("/users/PAS2136/alyeast/Beetle/DataVis/PUUM_bodysize_all.png", width = 12, height = 6, units = "in", res = 300)# Set output file and resolution
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png("./PUUM_bodysize_all.png", width = 12, height = 6, units = "in", res = 300)# Set output file and resolution
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ggplot(data = body_size, aes(x = cm_elytra_max_length, fill = Species)) +
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geom_density(alpha = 0.5) +
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xlab("Elytra Length (cm)") +
@@ -300,7 +300,7 @@ body_size_NEON<-merge(body_size, combined_data, by="individualID", all.x = TRUE,
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body_size_NEON<-subset(body_size_NEON, plotID!="PUUM_016")
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png("/users/PAS2136/alyeast/Beetle/DataVis/PUUM_bodysize_all.png", width = 12, height = 6, units = "in", res = 300)# Set output file and resolution
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png("./PUUM_bodysize_all.png", width = 12, height = 6, units = "in", res = 300)# Set output file and resolution
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ggplot(data = body_size_NEON, aes(x = cm_elytra_max_length, fill = scientificName)) +
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geom_density(alpha = 0.5) +
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xlab("Elytra Length (cm)") +
@@ -318,12 +318,12 @@ legend <- get_legend(density_plot)
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library(grid)
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library(gridExtra)
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png("/users/PAS2136/alyeast/Beetle/DataVis/PUUM_bodysize_legend.png", width = 2.5, height = 4, units = "in", res = 300, bg = "transparent")# Set output file and resolution
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png("./PUUM_bodysize_legend.png", width = 2.5, height = 4, units = "in", res = 300, bg = "transparent")# Set output file and resolution
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grid.newpage()
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grid.draw(legend)
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dev.off()
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png("/users/PAS2136/alyeast/Beetle/DataVis/PUUM_bodysize_all_wrap_4.png", width = 9, height = 9, units = "in", res = 300)# Set output file and resolution
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png("./PUUM_bodysize_all_wrap_4.png", width = 9, height = 9, units = "in", res = 300)# Set output file and resolution
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ggplot(data = body_size_NEON, aes(x = cm_elytra_max_length, fill = scientificName)) +
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geom_density(alpha = 0.5) +
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xlab("Elytra Length (cm)") +
@@ -342,7 +342,7 @@ Plot_locations<-as.data.frame(Plot_locations)
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Plot_locations<-Plot_locations[-c(10),]
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Plot_locations
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write.csv(Plot_locations, "/users/PAS2136/alyeast/Beetle/DataVis/Plot_locations.csv")
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write.csv(Plot_locations, "./Plot_locations.csv")
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# 2. Subset to species with >10 individuals
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species_to_keep <- names(species_counts[species_counts > 10])
@@ -368,7 +368,7 @@ body_size<-subset(body_size, length_cm>0)
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# 5. Plot
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png("/users/PAS2136/alyeast/Beetle/DataVis/PUUM_bodysize.png", width = 12, height = 6, units = "in", res = 300)# Set output file and resolution
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png("./PUUM_bodysize.png", width = 12, height = 6, units = "in", res = 300)# Set output file and resolution
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ggplot(data = body_size_filtered, aes(x = length_cm, fill = Species)) +
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geom_density(alpha = 0.5) +
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xlab("Elytra Length (cm)") +
@@ -379,7 +379,7 @@ dev.off()
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min(body_size$length_cm)
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png("/users/PAS2136/alyeast/Beetle/DataVis/PUUM_bodysize_all.png", width = 10, height = 10, units = "in", res = 300)# Set output file and resolution
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png("./PUUM_bodysize_all.png", width = 10, height = 10, units = "in", res = 300)# Set output file and resolution
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ggplot(data = body_size, aes(x=length_cm, fill = Species)) +
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geom_density(alpha=0.5) +
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theme_pubr() + # Apply publication-style theme
@@ -420,4 +420,4 @@ site_summary<-as.data.frame(site_summary)
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site_summary<-rbind(site_summary, c("PUUM", n_distinct(unique_beetle$Species), nrow(unique_beetle)))
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tail(site_summary)
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write.csv(site_summary, "/users/PAS2136/alyeast/Beetle/DataVis/site_summary.csv")
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write.csv(site_summary, "./site_summary.csv")

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