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source/content/Dry/Image Processing/Imaris 3D Random Spots Distance Analysis.md

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@@ -18,11 +18,11 @@ This Imaris XT Python workflow performs a 3D null model analysis by randomly gen
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| ObservedSpots | Real experimental spot set to compare against |
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### 🛠 Required Scripts
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| ⚙️ Script | 🔧 Purpose |
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| ----------------------------------------------------------------------------------------------------------------------------------------------------------- | ----------------------------------------------- |
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| `ImarisLib.py` | Interface to Imaris XT and image access |
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| [`Imaris_Analysis-RandomSpots.ipynb`](https://raw.githubusercontent.com/LabOnoM/DK.BeesGO/master/source/content/01.Files/Imaris_Analysis-RandomSpots.ipynb) | Main script for seeding, sampling, and analysis |
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![Workflow-of-CID-mapping](https://raw.githubusercontent.com/LabOnoM/DK.BeesGO/master/source/content/00.Images/Workflow-of-CID-mapping.png)
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| ⚙️ Script | 🔧 Purpose |
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| ----------------------------------------------------------------------------------- | ----------------------------------------------- |
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| `ImarisLib.py` | Interface to Imaris XT and image access |
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| [`Imaris_Analysis-RandomSpots.ipynb`](./01.Files/Imaris_Analysis-RandomSpots.ipynb) | Main script for seeding, sampling, and analysis |
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### 🧭 Workflow Summary
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1. Connects to an open Imaris application using ImarisLib.
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2. Make a distance map of your target signal by using the "Distance Transformation" from Image Processing -> Surfaces Functions -> Distance Transformation (requires XTension); name this channel as "distance."

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