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Step 5 : Compute PSTH

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Venus Sherathiya edited this page Aug 4, 2021 · 9 revisions

  1. In the jupyter notebook, run the cell corresponding to Data Analysis.

  2. After execution of this step, PSTH will be computed for each event. The transients detection in z-score or/and ΔF/F will be carried out too.

  3. The peak and area under the curve in the PSTH mean trace for each event will be computed too.

  4. The peak is found between the peak parameters set by the user in the Input Parameters GUI.

  5. Area under curve for the PSTH mean trace is computed using the trapezoidal method, beginning from the start time (user-defined) till the user-defined end point time.

  6. All the outputs in this step are either saved in 'csv' or 'h5' format which makes it easy for user to access it in other programming languages.

  7. For Group Analysis (average of multiple recording sessions), select all the folders for which you want to take the average in the Group Analysis section of Input Parameters GUI. Set the parameter Average Group? to True and run the cell corresponding to Data Analysis again.

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