diff --git a/testsuite/MDAnalysisTests/core/test_atomselections.py b/testsuite/MDAnalysisTests/core/test_atomselections.py
index 214b97e1ce..c00282fdcf 100644
--- a/testsuite/MDAnalysisTests/core/test_atomselections.py
+++ b/testsuite/MDAnalysisTests/core/test_atomselections.py
@@ -33,6 +33,7 @@
 from MDAnalysis import SelectionError, SelectionWarning
 from MDAnalysis.core.selection import Parser
 from MDAnalysis.lib.distances import distance_array
+from MDAnalysis.lib.pkdtree import PeriodicKDTree
 from MDAnalysis.tests.datafiles import (
     DCD,
     GRO,
@@ -57,6 +58,7 @@
 )
 from numpy.lib import NumpyVersion
 from numpy.testing import assert_equal
+from numpy.testing import assert_array_equal
 
 from MDAnalysisTests import make_Universe
 
@@ -1774,3 +1776,32 @@ def test_formal_charge_selection(sel, size, name):
 
     assert len(ag) == size
     assert ag.atoms[0].name == name
+
+
+def test_cylayer_selection_matches_periodic_kdtree():
+    u = mda.Universe(PSF, DCD)
+    u.dimensions = np.array([100.0, 100.0, 100.0, 90.0, 90.0, 90.0])
+
+    sel = u.select_atoms("cylayer 5 10 15 -15 name CA")
+
+    ref = u.select_atoms("name CA")
+    center = ref.center_of_geometry()
+
+    inner, outer, zmax, zmin = 5.0, 10.0, 15.0, -15.0
+    search_radius = np.sqrt(outer**2 + zmax**2)
+
+    kdtree = PeriodicKDTree(u.dimensions)
+    kdtree.set_coords(u.atoms.positions, cutoff=search_radius)
+
+    idx = kdtree.search(center, search_radius)
+
+    pos = u.atoms.positions[idx]
+    dxy = np.sqrt((pos[:, 0] - center[0]) ** 2 + (pos[:, 1] - center[1]) ** 2)
+    dz = pos[:, 2] - center[2]
+
+    mask = (inner <= dxy) & (dxy <= outer) & (zmin <= dz) & (dz <= zmax)
+
+    expected = np.sort(idx[mask])
+    result = np.sort(sel.indices)
+
+    assert_array_equal(result, expected)