@@ -21,15 +21,19 @@ install.packages('primerTree')
2121#### Github ####
2222``` s
2323# install.packages("devtools")
24- devtools::install_github("jimhester /primerTree")
24+ devtools::install_github("mvesuviusc /primerTree")
2525```
2626### [ Clustal Omega] ( http://www.clustal.org/omega/#Download ) Installation ###
2727#### Windows ####
2828Use the pre-compiled windows binary. Either put the installed clustalo.exe in your path, or pass the path to the executable in the clustal_options option
2929``` s
3030library(primerTree)
31- mammals_16S = search_primer_pair(name='Mammals 16S',
32- 'CGGTTGGGGTGACCTCGGA', 'GCTGTTATCCCTAGGGTAACT', clustal_options=c(exec='C:\Program Files\Clustal Omega\clustalo.exe'))
31+ mammals_16S <- search_primer_pair(
32+ name = 'Mammals 16S',
33+ 'CGGTTGGGGTGACCTCGGA',
34+ 'GCTGTTATCCCTAGGGTAACT',
35+ clustal_options = c(exec = 'C:\Program Files\Clustal Omega\clustalo.exe')
36+ )
3337```
3438#### Linux ####
3539Simple installation from source
@@ -62,7 +66,11 @@ From the clustalo directory:
6266Simple search for a Mammal 16S primer
6367``` s
6468library(primerTree)
65- mammals_16S = search_primer_pair(name='Mammals 16S', 'CGGTTGGGGTGACCTCGGA', 'GCTGTTATCCCTAGGGTAACT')
69+ mammals_16S <- search_primer_pair(
70+ name = 'Mammals 16S',
71+ 'CGGTTGGGGTGACCTCGGA',
72+ 'GCTGTTATCCCTAGGGTAACT'
73+ )
6674plot(mammals_16S)
6775```
6876
@@ -71,7 +79,11 @@ Using the parallel features with the multicore package using the doMC backend, w
7179library(doMC)
7280registerDoMC(8)
7381library(primerTree)
74- mammals_16S = search_primer_pair(name='Mammals 16S',
75- 'CGGTTGGGGTGACCTCGGA', 'GCTGTTATCCCTAGGGTAACT', .parallel=T)
82+ mammals_16S <- search_primer_pair(
83+ name = 'Mammals 16S',
84+ 'CGGTTGGGGTGACCTCGGA',
85+ 'GCTGTTATCCCTAGGGTAACT',
86+ .parallel = TRUE
87+ )
7688plot(mammals_16S)
7789```
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