Error: The DIA-NN output files necessary for the visualization are not found.

[rlumpkin]

I added the paths to the full DiaNN output folder, the raw file folder, and the full path to the fasta file interface to AlphaViz 1.1.15 GUI web interface. The Raw File Loads, and chromatograms are displayed, but AlphaViz returns the error "The DIA-NN output files necessary for the visualization are not found.". I tried this with two different datasets from DiaNN 1.8 to the same effect. The folders have the full DiaNN output in them.

MacOS Log
Last login: Fri Feb 10 11:44:49 on ttys000
/Applications/alphaviz.app/Contents/MacOS/alphaviz_gui ; exit;
redacted ~ % /Applications/alphaviz.app/Contents/MacOS/alphaviz_gui ; exit;
WARNING:root:WARNING: No Bruker libraries are available for this operating system. Intensities are uncalibrated, resulting in (very) small differences. However, mobility and m/z values need to be estimated. While this estimation often returns acceptable results with errors < 0.02 Th, huge errors (e.g. offsets of 6 Th) have already been observed for some samples!
Current AlphaViz version is up-to-date with GitHub.
Launching server at http://localhost:54017
WARNING:root:WARNING: No Bruker libraries are available for this operating system. Intensities are uncalibrated, resulting in (very) small differences. However, mobility and m/z values need to be estimated. While this estimation often returns acceptable results with errors < 0.02 Th, huge errors (e.g. offsets of 6 Th) have already been observed for some samples!
Reading the DIA-NN output files...
2023-02-10 14:32:57> Importing data from redacted/Desktop/input/esf4_0058_DMSO_Slot2-28_1_139.d
2023-02-10 14:32:57> Reading frame metadata for redacted/Desktop/input/esf4_0058_DMSO_Slot2-28_1_139.d
2023-02-10 14:32:58> Reading 64,275 frames with 2,589,379,016 detector events for redacted/Desktop/input/esf4_0058_DMSO_Slot2-28_1_139.d
2023-02-10 14:34:29> Indexing redacted/Desktop/input/esf4_0058_DMSO_Slot2-28_1_139.d...
2023-02-10 14:34:29> Bruker DLL not available, estimating mobility values
2023-02-10 14:34:29> Bruker DLL not available, estimating mz values
2023-02-10 14:34:29> Indexing quadrupole dimension
2023-02-10 14:34:54> Succesfully imported data from redacted/Desktop/input/esf4_0058_DMSO_Slot2-28_1_139.d
Reading the DIA-NN output files...

Windows Log
Current AlphaViz version is up-to-date with GitHub.
Launching server at http://localhost:54438
Reading the DIA-NN output files...

Version

• Installation type = GUI release
• system = MacOS 12.6.3 and Windows 10
• version = AlphaViz 1.1.15
• machine = Intel iMac and x86_64 PC