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docs/README.rst

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AdapterRemoval v2
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=================
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.. image:: https://img.shields.io/travis/MikkelSchubert/adapterremoval/master.svg
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:target: https://travis-ci.org/MikkelSchubert/adapterremoval
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:alt: Travis-CI
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.. image:: https://img.shields.io/coveralls/MikkelSchubert/adapterremoval.svg
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:target: https://coveralls.io/github/MikkelSchubert/adapterremoval
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:alt: Coveralls
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AdapterRemoval searches for and removes adapter sequences from High-Throughput
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Sequencing (HTS) data and (optionally) trims low quality bases from the 3' end
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of reads following adapter removal. AdapterRemoval can analyze both single end
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and paired end data, and can be used to merge overlapping paired-ended reads
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into (longer) consensus sequences. Additionally, AdapterRemoval can construct a
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consensus adapter sequence for paired-ended reads, if which this information is
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not available.
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If you use AdapterRemoval v2, then please cite the paper
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Schubert, Lindgreen, and Orlando (2016). AdapterRemoval v2: rapid adapter
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trimming, identification, and read merging. BMC Research Notes, 12;9(1):88
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http://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-016-1900-2
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AdapterRemoval was originally published in Lindgreen 2012:
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Lindgreen (2012): AdapterRemoval: Easy Cleaning of Next Generation
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Sequencing Reads, BMC Research Notes, 5:337
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http://www.biomedcentral.com/1756-0500/5/337/
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Overview of major features
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==========================
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- Trimming of adapters sequences from single-end and paired-end FASTQ reads.
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- Trimming of multiple, different adapters or adapter pairs.
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- Demultiplexing of single or double indexed reads, with or without trimming
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of adapter sequences.
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- Reconstruction of adapter sequences from paired-end reads, by the pairwise
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alignment of reads in the absence of a known adapter sequence.
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- Merging of overlapping read-pairs into higher-quality consensus sequences.
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- Multi-threading of all operations for increased throughput.
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- Reading and writing of gzip and bzip2 compressed files.
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- Reading and writing of interleaved FASTQ files.
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Installation
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============
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For detailed installation instructions, please see the
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`Installation <https://adapterremoval.readthedocs.io/en/latest/installation.html>`_
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section of the online documentation.
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Installing AdapterRemoval using `Conda <https://conda.io/docs/>`_
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-----------------------------------------------------------------
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If you have Conda `installed on your system <https://conda.io/miniconda.html>`_,
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you may install AdapterRemoval using one of serveral unoffical recipies::
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conda install -c bioconda adapterremoval
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conda install -c maxibor adapterremoval2
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Installing AdapterRemoval on Debian based systems
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-------------------------------------------------
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Users of Debian Stretch or Ubuntu Artful Aardvark may install AdapterRemoval
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using Apt::
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sudo apt-get install adapterremoval adapterremoval-examples
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Building AdapterRemoval from scratch
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------------------------------------
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Compiling AdapterRemoval from scratch requires a C++11 compliant compiler, and
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that the zlib and bz2lib headers are installed::
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make
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sudo make install
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Getting started
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===============
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To run AdapterRemoval, specify the location of pair 1 and (optionally) pair 2
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FASTQ using the --file1 and --file2 command-line options::
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AdapterRemoval --file1 myreads_1.fastq.gz --file2 myreads_2.fastq.gz
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By default, AdapterRemoval will save the trimmed reads in the current working
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directly, using filenames starting with 'your_output'. See the
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`Input and Output
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<https://adapterremoval.readthedocs.io/en/latest/input_and_output.html>`_
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files section for more information about files generated by AdapterRemoval.
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More examples of common usage may be found in the
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`Examples <https://adapterremoval.readthedocs.io/en/latest/examples.html>`_
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section of the online documentation.
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Documentation
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-------------
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For a detailed description of program usage, please refer to the online
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`online documentation <https://adapterremoval.readthedocs.io/>`_.

docs/input_and_output.rst

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Input and output files
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======================
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Output files
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------------

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