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| 1 | +AdapterRemoval v2 |
| 2 | +================= |
| 3 | + |
| 4 | +.. image:: https://img.shields.io/travis/MikkelSchubert/adapterremoval/master.svg |
| 5 | + :target: https://travis-ci.org/MikkelSchubert/adapterremoval |
| 6 | + :alt: Travis-CI |
| 7 | + |
| 8 | +.. image:: https://img.shields.io/coveralls/MikkelSchubert/adapterremoval.svg |
| 9 | + :target: https://coveralls.io/github/MikkelSchubert/adapterremoval |
| 10 | + :alt: Coveralls |
| 11 | + |
| 12 | +AdapterRemoval searches for and removes adapter sequences from High-Throughput |
| 13 | +Sequencing (HTS) data and (optionally) trims low quality bases from the 3' end |
| 14 | +of reads following adapter removal. AdapterRemoval can analyze both single end |
| 15 | +and paired end data, and can be used to merge overlapping paired-ended reads |
| 16 | +into (longer) consensus sequences. Additionally, AdapterRemoval can construct a |
| 17 | +consensus adapter sequence for paired-ended reads, if which this information is |
| 18 | +not available. |
| 19 | + |
| 20 | +If you use AdapterRemoval v2, then please cite the paper |
| 21 | + |
| 22 | + Schubert, Lindgreen, and Orlando (2016). AdapterRemoval v2: rapid adapter |
| 23 | + trimming, identification, and read merging. BMC Research Notes, 12;9(1):88 |
| 24 | + http://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-016-1900-2 |
| 25 | + |
| 26 | +AdapterRemoval was originally published in Lindgreen 2012: |
| 27 | + |
| 28 | + Lindgreen (2012): AdapterRemoval: Easy Cleaning of Next Generation |
| 29 | + Sequencing Reads, BMC Research Notes, 5:337 |
| 30 | + http://www.biomedcentral.com/1756-0500/5/337/ |
| 31 | + |
| 32 | + |
| 33 | +Overview of major features |
| 34 | +========================== |
| 35 | + |
| 36 | + - Trimming of adapters sequences from single-end and paired-end FASTQ reads. |
| 37 | + - Trimming of multiple, different adapters or adapter pairs. |
| 38 | + - Demultiplexing of single or double indexed reads, with or without trimming |
| 39 | + of adapter sequences. |
| 40 | + - Reconstruction of adapter sequences from paired-end reads, by the pairwise |
| 41 | + alignment of reads in the absence of a known adapter sequence. |
| 42 | + - Merging of overlapping read-pairs into higher-quality consensus sequences. |
| 43 | + - Multi-threading of all operations for increased throughput. |
| 44 | + - Reading and writing of gzip and bzip2 compressed files. |
| 45 | + - Reading and writing of interleaved FASTQ files. |
| 46 | + |
| 47 | + |
| 48 | +Installation |
| 49 | +============ |
| 50 | + |
| 51 | +For detailed installation instructions, please see the |
| 52 | +`Installation <https://adapterremoval.readthedocs.io/en/latest/installation.html>`_ |
| 53 | +section of the online documentation. |
| 54 | + |
| 55 | +Installing AdapterRemoval using `Conda <https://conda.io/docs/>`_ |
| 56 | +----------------------------------------------------------------- |
| 57 | + |
| 58 | +If you have Conda `installed on your system <https://conda.io/miniconda.html>`_, |
| 59 | +you may install AdapterRemoval using one of serveral unoffical recipies:: |
| 60 | + |
| 61 | + conda install -c bioconda adapterremoval |
| 62 | + conda install -c maxibor adapterremoval2 |
| 63 | + |
| 64 | + |
| 65 | +Installing AdapterRemoval on Debian based systems |
| 66 | +------------------------------------------------- |
| 67 | + |
| 68 | +Users of Debian Stretch or Ubuntu Artful Aardvark may install AdapterRemoval |
| 69 | +using Apt:: |
| 70 | + |
| 71 | + sudo apt-get install adapterremoval adapterremoval-examples |
| 72 | + |
| 73 | + |
| 74 | +Building AdapterRemoval from scratch |
| 75 | +------------------------------------ |
| 76 | + |
| 77 | +Compiling AdapterRemoval from scratch requires a C++11 compliant compiler, and |
| 78 | +that the zlib and bz2lib headers are installed:: |
| 79 | + |
| 80 | + make |
| 81 | + sudo make install |
| 82 | + |
| 83 | + |
| 84 | +Getting started |
| 85 | +=============== |
| 86 | + |
| 87 | +To run AdapterRemoval, specify the location of pair 1 and (optionally) pair 2 |
| 88 | +FASTQ using the --file1 and --file2 command-line options:: |
| 89 | + |
| 90 | + AdapterRemoval --file1 myreads_1.fastq.gz --file2 myreads_2.fastq.gz |
| 91 | + |
| 92 | +By default, AdapterRemoval will save the trimmed reads in the current working |
| 93 | +directly, using filenames starting with 'your_output'. See the |
| 94 | +`Input and Output |
| 95 | +<https://adapterremoval.readthedocs.io/en/latest/input_and_output.html>`_ |
| 96 | +files section for more information about files generated by AdapterRemoval. |
| 97 | + |
| 98 | +More examples of common usage may be found in the |
| 99 | +`Examples <https://adapterremoval.readthedocs.io/en/latest/examples.html>`_ |
| 100 | +section of the online documentation. |
| 101 | + |
| 102 | + |
| 103 | +Documentation |
| 104 | +------------- |
| 105 | + |
| 106 | +For a detailed description of program usage, please refer to the online |
| 107 | +`online documentation <https://adapterremoval.readthedocs.io/>`_. |
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