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Exercises/Exercise6_NeuroNLP.md renamed to Exercises/Exercise6_FruitFlyBrainObservatory.md

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# Exploring Brain Circuits with the Fruit Fly Brain Observatory
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This file contains a few quick tutorial on how to get started exploring the fruit fly brain using the [Fruit Fly Brain Observatory](http://fruitflybrain.org/) platform. This tutorial contains the following sections:
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- Exploratory Circuit Discovery with NeuroNLP
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- [Exercise A: Access NeuroNLP and view the Demos](#Access-NeuroNLP-and-view-the-Demos)
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- [Exercise B: Visualize Neurons in the Ellipsoid body](#Visualize-Neurons-in-the-Ellipsoid-body)
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- [Exercise C: Explore Fly Medulla Circuit from Janelia EM Data](#Explore-Fly-Medulla-Circuit-from-Janelia-EM-Data)
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- [Exercise D: Create and share a Tag](#Create-and-share-a-Tag)
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- [Exercise E: Explore Networks from recent Publications](Explore-Networks-from-recent-Publications)
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- Exploring Brain Circuit Function at Multiple Levels
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- [TODO]
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## Exploratory Circuit Discovery with NeuroNLP
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This contains a quick tutorial on how to get started exploring the fruit fly brain with [NeuroNLP](https://neuronlp.fruitflybrain.org/), which is part of the [Fruit Fly Brain Observatory](http://fruitflybrain.org/) platform.
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This section contains a few demos on how to get started exploring the fruit fly brain with [NeuroNLP](https://neuronlp.fruitflybrain.org/), which is part of the [Fruit Fly Brain Observatory](http://fruitflybrain.org/) platform.
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### Local software requirements
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There are lots of ways you can explore the data in NeuroNLP, check out the demos for more ways to slice and combine the data.
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#### Explore the Lamina and build up a Cartridge model
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#### Explore Fly Medulla Circuit from Janelia EM Data
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NeuroNLP contains the detailed connectomic data of the visual system. We can start out by visualizing all the Lamina neurons in NeuroNLP
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NeuroNLP contains the detailed connectomic data of the [7 column medulla Electro-Microscopy (EM) data](https://github.com/janelia/connectomehackthon2015) published by Janelia research campus. We can start out by visualizing all the Lamina neurons that innervate the Medulla in NeuroNLP
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"Show neurons in the Lamina"
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We can look at the first layer of neurons in the lamina, the L1 neurons, using:
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Note that the data only contains the part of the neurons in the medulla. Therefore, currently, we can only visualize their axons in the Medulla.
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We can look at the axons of the Lamina L1 neurons in the Medulla, using:
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"Show L1 neurons in the lamina"
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Here we can start to see the columnar nature of the Lamina. We can further look at only a single cartridge in the lamina by using:
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Nevertheless we can start to see the columnar nature of the Lamina. We can further look at only a single cartridge in the lamina by using:
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"Show neurons in a single cartridge in the lamina"
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We can also start to build up the flow of connectivity in the Lamina, by starting with a single L1 neuron.
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"Show L1 neurons in a single cartridge in the lamina"
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As we are using the connectomic data, we can start to build up the circuit connectomically, by adding the post synaptic neurons:
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As we are using the connectomic data, we can start to build up the circuit connectomically, by adding the postsynaptic Mi1 and Tm3 neurons:
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"Add post synaptic neurons"
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"Add postsynaptic Mi1 neurons"
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"Add postsynaptic Tm3 neurons"
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We can see a growing circuit of 13 neurons directly connected to the single L1 neuron. We can further run the same command, and build up the neurons 2 synaptic connections away, resulting in 246 neurons.
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From here we can pull back to look at the L2 neurons using
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In a recent paper by Sun Yi et. al., Neural signatures of dynamic stimulus selection in Drosophila, they describe a feedforward visual pathway from the medulla to the central complex. We can recreate and explore this network in NeuroNLP, by opening the following [10.1038/nn.4581](https://neuronlp.fruitflybrain.org/index.html?tag=10.1038/nn.4581) Tag.
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## Exploring Brain Circuit Function at Multiple Levels
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This section contains a few demos on how to explore executable brain circuits in [NeuroGFX](https://neurogfx.fruitflybrain.org/).
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<!-- ### Local software requirements
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None, apart from an up to date web browser -->
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### Instructions
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