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I have not had a chance to look at the file, but the fact that the NWB version is missing seems to indicate that the file is likely a NWB version 1.x file. PyNWB and MatNWB support NWB 2.0 and up (see https://nwb-overview.readthedocs.io/en/latest/faq.html#nwb-1-vs-2). I can take a look later to further confirm. If the file is indeed 1.x then you should still be able to open the file directly via HDF5.

Here a list of HDF5 tools that are useful inspect the raw HDF5 file https://nwb-overview.readthedocs.io/en/latest/tools/hdftools/hdftools.html#analysistools-hdftools In Python you can also use h5py directly to open the file.

import h5py
nwb_file = h5py.File("Markus Frey - R2470_experiment_1.…

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