Skip to content

Commit 0167663

Browse files
jwwojakecormany
andauthored
docs: update Flex robot and instrument cleaning instructions (#18902)
# Overview These changes prompted by customer misunderstanding calibration "recommendations" as "requirements." Trying to correct that in the cleaning instructions. Fixing any other code oddities. This one is simple and a low priority on the list. Getting to the end of the items. See RTC-647. ## Test Plan and Hands on Testing Check for code accuracy and grammar. ## Changelog Text changes to put emphasis on calibration as optional but recommended when re-attaching an instrument for cleaning. A "nice to have" but not required tone. ## Review requests n/a ## Risk assessment Low, documentation only --------- Co-authored-by: Ed Cormany <[email protected]>
1 parent a8a4778 commit 0167663

File tree

2 files changed

+112
-82
lines changed

2 files changed

+112
-82
lines changed

docs/flex-manual/docs/maintenance-service.md

Lines changed: 109 additions & 76 deletions
Original file line numberDiff line numberDiff line change
@@ -29,19 +29,19 @@ The following table lists the chemicals you can use to clean your Flex. Diluted
2929
!!! warning
3030
*Do not use acetone.* The robot, pipettes, and modules are made from materials that acetone can damage or dissolve.
3131

32-
| Solution | Recommendations |
33-
|------------|-----------------|
34-
| **Alcohol** | Includes ethyl/ethanol, isopropyl, and methanol. Dilute to 70% for cleaning. Do not use 100% alcohol. |
35-
| **Bleach** | Dilute to 10% (1:10 bleach/water ratio) for cleaning. Do not use 100% bleach. |
36-
| **Distilled water** | You can use distilled water to clean or rinse your robot. |
32+
| Solution | Recommendations |
33+
|----|----|
34+
| **Alcohol** | Includes ethyl/ethanol, isopropyl, and methanol. Dilute to 70% for cleaning. Do not use 100% alcohol. |
35+
| **Bleach** | Dilute to 10% (1:10 bleach/water ratio) for cleaning. Do not use 100% bleach. |
36+
| **Distilled water** | You can use distilled water to clean or rinse your robot. |
3737

3838
### Frame and window panel cleaning
3939

4040
To clean the exterior and interior frame and window panels of your Flex:
4141

4242
1. Dampen a soft, clean cloth or paper towel with a cleaning solution.
4343
2. Gently wipe off the exposed and easily accessible surface areas.
44-
3. Use a cloth dampened with distilled water for a rinse wipedown.
44+
3. Rinse off any remaining residue using a cloth dampened with distilled water.
4545
4. Let the robot air dry.
4646

4747
### Deck cleaning
@@ -50,16 +50,16 @@ To clean the deck, deck slots, and trash bin:
5050

5151
1. Dampen a soft, clean cloth or paper towel with a cleaning solution.
5252
2. Gently wipe off the deck, deck slots, and trash bin. You can remove the deck slots and trash bin for easier access.
53-
3. Use a cloth dampened with distilled water for a rinse wipedown.
54-
4. Let the deck pieces air dry. Replace any pieces that you removed for cleaning.
53+
3. Rinse off any remaining residue using a cloth dampened with distilled water.
54+
4. Let the deck pieces air dry. Replace any items that you removed for cleaning.
5555

5656
### Gantry cleaning
5757

5858
To clean the gantry:
5959

6060
1. Dampen a soft, clean cloth or paper towel with a cleaning solution.
6161
2. Gently wipe off the horizontal and vertical gantry surfaces, and side rails.
62-
3. Use a cloth dampened with distilled water for a rinse wipedown.
62+
3. Rinse off any remaining residue using a cloth dampened with distilled water.
6363
4. Let the gantry air dry.
6464

6565
### Waste chute cleaning
@@ -69,8 +69,9 @@ To clean the waste chute:
6969
1. Remove the waste chute from its deck plate adapter.
7070
2. Dampen a soft, clean cloth or paper towel with a cleaning solution.
7171
3. Gently wipe down the exterior of the chute. The interior is powder-coated steel, so you can clean it with mild detergents or surfactants.
72-
4. Use a cloth dampened with distilled water for a rinse wipedown.
73-
5. Let the waste chute air dry and reattach it to the deck.
72+
4. Rinse off any remaining residue using a cloth dampened with distilled water.
73+
5. Let the waste chute air dry.
74+
6. Reattach the waste chute to its deck plate adapter.
7475

7576
## Cleaning pipettes and tips
7677

@@ -82,8 +83,10 @@ To clean a 1-, 8-, or 96-channel pipette:
8283
- Body
8384
- Ejector
8485
- Nozzles
85-
4. Use a cloth dampened with distilled water for a rinse wipedown.
86-
5. Let the pipette air dry and reattach.
86+
4. Rinse off any remaining residue using a cloth dampened with distilled water.
87+
5. Let the pipette air dry.
88+
6. Reattach the pipette to the gantry. When prompted, note that recalibration is optional, but recommended.
89+
8790

8891
![Cleanable pipette components, including the body, ejector, and nozzles.](images/pipette-components-cleaning.png "Pipette components")
8992

@@ -111,10 +114,13 @@ Refer to the following table for recommended cleaning methods, by contamination
111114

112115
Filtered pipette tips help prevent contaminating the barrel or inside of the pipette. But, you cannot disassemble the barrel if it becomes contaminated. If the inside of your pipette gets contaminated, the following steps may help remove the contamination:
113116

114-
1. Inject a small amount of cleaning solution into the barrel using a manual pipette or syringe.
115-
2. Gently shake the pipette to swirl the cleaning solution.
116-
3. Rinse with distilled water.
117-
4. Let the pipette air dry and reattach.
117+
1. Remove the pipette from the gantry.
118+
2. Inject a small amount of cleaning solution into the barrel using a manual pipette or syringe.
119+
3. Gently shake the pipette to swirl the cleaning solution.
120+
4. Rinse with distilled water.
121+
5. Let the pipette air dry.
122+
6. Reattach the pipette to the gantry. When prompted, note that recalibration is optional, but recommended.
123+
118124

119125
### Cleaning pipette tips
120126

@@ -130,8 +136,9 @@ To clean the gripper:
130136
- Gripper body
131137
- Jaws
132138
- Paddles
133-
4. Use a cloth dampened with distilled water for a rinse wipedown.
134-
5. Let the gripper air dry and reattach.
139+
4. Rinse off any remaining residue using a cloth dampened with distilled water.
140+
5. Let the gripper air dry.
141+
6. Reattach the gripper to the gantry. When prompted, note that recalibration is optional, but recommended.
135142

136143
![Cleanable gripper components, including the body, jaws, and paddles.](images/gripper-components-cleaning.png "Gripper components"){width="50%"}
137144

@@ -167,7 +174,7 @@ Once you've prepared the module for cleaning:
167174

168175
1. Dampen a soft, clean cloth or paper towel with a cleaning solution.
169176
2. Gently wipe off the module's surfaces.
170-
3. Use a cloth dampened with distilled water as a rinse wipedown.
177+
3. Rinse off any remaining residue using a cloth dampened with distilled water.
171178
4. Let the module air dry.
172179

173180
### Thermocycler seals
@@ -185,12 +192,38 @@ To set up the Thermocycler with a clean seal:
185192

186193
The following table lists labware sold by Opentrons that we have verified as autoclave-safe. When you can't determine whether a piece of labware is autoclave-safe, just replace it with new, clean labware. You can [purchase replacement labware](https://opentrons.com/products/categories/tips-&-labware) from the Opentrons shop.
187194

188-
| Labware type | Autoclave-safe items |
189-
|--------------|----------------------|
190-
| **Reservoirs** | All NEST reservoirs |
191-
| **Sample vials** | Eppendorf Safe-Lock 1.5 mL and 2.0 mL vials (when left open at 121 °C, 20 min) |
192-
| **Tip racks and tips**| All Flex tip racks and tips |
193-
| **Well plates** | <ul><li>Thermo Scientific Nunc 96-Well Plate, 1300 μL</li><li>Thermo Scientific Nunc 96-Well Plate, 2000 μL</li><li>USA Scientific 96 Deep Well Plate, 2.4 mL</li></ul> |
195+
<table>
196+
<thead>
197+
<tr>
198+
<th>Labware type</th>
199+
<th>Autoclave-safe items</th>
200+
</tr>
201+
</thead>
202+
<tbody>
203+
<tr>
204+
<td><strong>Reservoirs</strong></td>
205+
<td>All NEST reservoirs</td>
206+
</tr>
207+
<tr>
208+
<td><strong>Sample vials</strong></td>
209+
<td>Eppendorf Safe-Lock 1.5 mL and 2.0 mL vials (when left open at 121 °C, 20 min)</td>
210+
</tr>
211+
<tr>
212+
<td><strong>Tip racks and tips</strong></td>
213+
<td>All Flex tip racks and tips</td>
214+
</tr>
215+
<tr>
216+
<td><strong>Well plates</strong></td>
217+
<td>
218+
<ul>
219+
<li>Thermo Scientific Nunc 96-Well Plate, 1300 μL</li>
220+
<li>Thermo Scientific Nunc 96-Well Plate, 2000 μL</li>
221+
<li>USA Scientific 96 Deep Well Plate, 2.4 mL</li>
222+
</ul>
223+
</td>
224+
</tr>
225+
</tbody>
226+
</table>
194227

195228
If you're using labware from a manufacturer that's not listed here, refer to their website to see whether those items can be autoclaved.
196229

@@ -212,7 +245,7 @@ In addition, Opentrons Care Plus includes on-site visits for:
212245
- Preventive maintenance, yearly.
213246
- Repairs, as needed.
214247

215-
You can also [purchase services](https://opentrons.com/instrument-services) a la carte, including installation, protocol development (remote or on-site), repair, relocation, and preventive maintenance. Contact Opentrons Sales for more information.
248+
You can also [purchase services](https://opentrons.com/instrument-services) individually, including installation, protocol development (remote or on-site), repair, relocation, and preventive maintenance. Contact Opentrons Sales for more information.
216249

217250
### Installation qualification and operation qualification
218251

@@ -227,55 +260,55 @@ Consult the [Flex IQ/OQ form](https://opentrons-flex-iq-oq-checklist.paperform.c
227260

228261
### Preventative maintenance
229262

230-
Opentrons recommends performing basic maintenance tasks — above and beyond cleaning, but not repairs or service — on a regular schedule. These recommendations assume that Flex is in operation 20 hours per week, 50 weeks per year. Adjust the schedule to meet your needs if you use your Flex more or less.
231-
232-
<table markdown>
233-
<thead>
234-
<tr>
235-
<th>Frequency</th>
236-
<th>Task</th>
237-
<th>Description</th>
238-
</tr>
239-
</thead>
240-
<tbody markdown>
241-
<tr>
242-
<td rowspan="2">Daily</td>
243-
<td>Empty trash</td>
244-
<td>Take the movable trash out of its deck slot, safely discard its contents, and replace it into the deck.</td>
245-
</tr>
246-
<tr>
247-
<td>Inspect working area</td>
248-
<td>Clear the deck of any debris, liquid, or no-longer-needed labware. Check that the plastic labware clips are not damaged.</td>
249-
</tr>
250-
<tr>
251-
<td>Weekly</td>
252-
<td>Clean surfaces</td>
253-
<td>Clean the exterior surfaces of the deck, gantry, windows, instruments, and other hardware, as described above.</td>
254-
</tr>
255-
<tr>
256-
<td>Monthly</td>
257-
<td>Power cycle</td>
258-
<td>Turn off Flex and all connected modules. Then turn them back on.</td>
259-
</tr>
260-
<tr>
261-
<td rowspan="3">Every six months</td>
262-
<td>Inspect pipettes</td>
263-
<td>Inspect the O-rings on the pipette nozzles for signs of wear (notches, deformation). Replace the O-rings with the provided spares, if needed.</td>
264-
</tr>
265-
<tr>
266-
<td>Inspect gripper</td>
267-
<td>Inspect the rubber pads on the gripper paddles. Replace the gripper paddles with the provided spares, if needed.</td>
268-
</tr>
269-
<tr>
270-
<td>Recalibrate instruments</td>
271-
<td>Run recalibration for the pipettes and gripper.</td>
272-
</tr>
273-
<tr markdown>
274-
<td>Yearly</td>
275-
<td>Evaluate pipette performance</td>
276-
<td markdown>Evaluate the overall performance of your Flex pipettes. Replace any pipette that is not performing according to the [published accuracy and precision standards][pipette-specifications].</td>
277-
</tr>
278-
</tbody>
263+
Along with routine cleaning, Opentrons also recommends the following optional procedures to help keep your Flex running smoothly. These suggestions are based on a Flex that operates for about 20 hours per week, 50 weeks per year. Feel free to adapt this schedule to your robot's workload. Alternatively, let Opentrons do the work for you—see the [Instrument Services section](https://opentrons.com/instrument-services) of our website for information about our service contract offerings.
264+
265+
<table>
266+
<thead>
267+
<tr>
268+
<th>Frequency</th>
269+
<th>Task</th>
270+
<th>Description</th>
271+
</tr>
272+
</thead>
273+
<tbody>
274+
<tr>
275+
<td rowspan="2">Daily</td>
276+
<td>Empty trash</td>
277+
<td>Take the movable trash out of its deck slot, safely discard its contents, and replace it into the deck.</td>
278+
</tr>
279+
<tr>
280+
<td>Inspect working area</td>
281+
<td>Clear the deck of any debris, liquid, or no-longer-needed labware. Check that the plastic labware clips are not damaged.</td>
282+
</tr>
283+
<tr>
284+
<td>Weekly</td>
285+
<td>Clean surfaces</td>
286+
<td>Clean the exterior surfaces of the deck, gantry, windows, instruments, and other hardware, as described above.</td>
287+
</tr>
288+
<tr>
289+
<td>Monthly</td>
290+
<td>Power cycle</td>
291+
<td>Turn off Flex and all connected modules. Then turn them back on.</td>
292+
</tr>
293+
<tr>
294+
<td rowspan="3">Every six months</td>
295+
<td>Inspect pipettes</td>
296+
<td>Inspect the O-rings on the pipette nozzles for signs of wear (notches, deformation). Replace the O-rings with the provided spares, if needed.</td>
297+
</tr>
298+
<tr>
299+
<td>Inspect gripper</td>
300+
<td>Inspect the rubber pads on the gripper paddles. Replace the gripper paddles with the provided spares, if needed.</td>
301+
</tr>
302+
<tr>
303+
<td>Recalibrate instruments</td>
304+
<td>Run recalibration for the pipettes and gripper.</td>
305+
</tr>
306+
<tr>
307+
<td>Yearly</td>
308+
<td>Evaluate pipette performance</td>
309+
<td>Evaluate the overall performance of your Flex pipettes. Replace any pipette that is not performing according to the <a href="../system-description/#pipette-specifications">published accuracy and precision standards</a>.</td>
310+
</tr>
311+
</tbody>
279312
</table>
280313

281314
The annual on-site preventative maintenance visit that is part of Opentrons Care Plus includes performance of all of the tasks listed above, as well as replacement parts.

docs/flex-manual/docs/system-description.md

Lines changed: 3 additions & 6 deletions
Original file line numberDiff line numberDiff line change
@@ -140,7 +140,7 @@ The primary user interface is the 7-inch LCD *touchscreen*, located on the front
140140

141141
- Operation logs and error notifications
142142

143-
For more information on using Flex via the touchscreen, see the of the Software and Operation chapter.
143+
For more information on using Flex via the touchscreen, see the [Touchscreen Operation section](software-operation.md#touchscreen-operation) of the Software and Operation chapter.
144144

145145
The *status light* is a strip of LEDs along the top front of the robot that provides at-a-glance information about the robot. Different colors and patterns of illumination can communicate various success, failure, or idle states:
146146

@@ -219,10 +219,7 @@ The pipettes pick up disposable plastic *tips* by pressing them onto the pipette
219219
### Pipette specifications
220220

221221
Opentrons Flex pipettes are designed to handle a wide range of volumes.
222-
Because of their wide overall range, they can use multiple sizes of
223-
tips, which affect their liquid-handling characteristics. Opentrons has
224-
tested Flex pipettes for accuracy and precision in a number of tip and
225-
liquid volume combinations:
222+
Because of their wide overall range, they can use multiple sizes of tips, which affect their liquid-handling characteristics. Opentrons has tested Flex pipettes for accuracy and precision in a number of tip and liquid volume combinations:
226223

227224
<table>
228225
<thead>
@@ -356,7 +353,7 @@ pipette. In general, for best results you should use the smallest tips
356353
that meet the needs of your protocol.
357354

358355
!!! note
359-
Opentrons performs volumetric testing of Flex pipettes to ensure that they meet the accuracy and precision specifications listed above. You *do not* have to calibrate the volume that your pipettes dispense before use. You only have to perform positional calibration. See the next section, as well as the of the [Pipette Installation section][pipette-installation] Installation and Relocation chapter, for details.
356+
Opentrons performs volumetric testing of Flex pipettes to ensure that they meet the accuracy and precision specifications listed above. You *do not* have to calibrate the volume that your pipettes dispense before use. You only have to perform positional calibration. See the next section, as well as the [Pipette Installation section][pipette-installation] of the Installation and Relocation chapter, for details.
360357

361358
The Opentrons Care and Opentrons Care Plus services include yearly pipette replacement and certificates of calibration. See the [Servicing Flex section][servicing-flex] of the Maintenance and Service chapter for details.
362359

0 commit comments

Comments
 (0)