@@ -102,14 +102,21 @@ Parameters for the complex liquid handling are listed here in order of operation
102
102
+--------------------------------+------------------------------------------------------+----------------------------+------------------------------------+------------------------------------+
103
103
| ``air_gap `` | Volume in µL | 0 | 0 | 0 |
104
104
+--------------------------------+------------------------------------------------------+----------------------------+------------------------------------+------------------------------------+
105
- | ``blow_out `` | ``True `` or ``False ``, if true blow out at dispense | ``False `` | ``False `` | ``False `` |
105
+ | ``blow_out `` | ``True `` or ``False ``, if true and no location | ``False `` | ``False `` | ``False `` |
106
+ | | specified it will blow out in the trash. | | | |
107
+ | | | | | |
108
+ | | **Note **: | | | |
109
+ | | 1. If the pipette tip is empty, and no location is | | | |
110
+ | | specified, the pipette will blow out in the trash. | | | |
111
+ | | 2. If the pipette tip is not empty, and no | | | |
112
+ | | location is specified, the pipette will blow out | | | |
113
+ | | into the source well. | | | |
106
114
+--------------------------------+------------------------------------------------------+----------------------------+------------------------------------+------------------------------------+
107
- | ``blowout_location `` | ``trash ``, ``source well ``, ``destination well `` | If pipette empty, blowout | If pipette empty, blowout | If pipette empty, blowout |
108
- | | | into trash, otherwise | into trash, otherwise | into trash, otherwise |
109
- | | | blowout into source well | blowout into source well | blowout into source well |
110
- | | | If ``blow_out `` is false | If ``blow_out `` is false | If ``blowout `` is false |
111
- | | | ``blowout_location `` will | ``blowout_location `` will | ``blowout_location `` will |
112
- | | | be ignored | be ignored | be ignored |
115
+ | ``blowout_location `` | ``trash ``, ``source well ``, ``destination well `` | There is no location by | There is no location by | There is no location by |
116
+ | | | default. Please see | default. Please see ``blow_out `` | default. Please see ``blow_out `` |
117
+ | | **Note **: If ``blow_out `` is set to ``False `` this | ``blow_out `` above for | above for default behavior. | above for default behavior. |
118
+ | | parameter will be ignored. | default behavior. | | |
119
+ | | | | | |
113
120
+--------------------------------+------------------------------------------------------+----------------------------+------------------------------------+------------------------------------+
114
121
| ``trash `` | ``True `` or ``False ``, if false return tip to tiprack| ``True `` | ``True `` | ``True `` |
115
122
+--------------------------------+------------------------------------------------------+----------------------------+------------------------------------+------------------------------------+
@@ -727,7 +734,7 @@ will have the steps...
727
734
``blow_out ``
728
735
------------
729
736
730
- A :ref: `blow-out ` can be performed after every dispense that leaves the tip empty by setting ``blow_out=True ``.
737
+ A :ref: `blow-out ` into the trash can be performed after every dispense that leaves the tip empty by setting ``blow_out=True ``.
731
738
732
739
.. code-block :: python
733
740
@@ -751,6 +758,80 @@ will have the steps...
751
758
752
759
.. versionadded :: 2.0
753
760
761
+ The robot will automatically dispense any left over liquid that is *not * from using ``disposal_volume `` into
762
+ the source well of your transfer function.
763
+
764
+ .. code-block :: python
765
+
766
+ pipette.pick_up_tip()
767
+ pipette.aspirate(10 , plate[' A1' ])
768
+ pipette.transfer(
769
+ 100 ,
770
+ plate[' A1' ],
771
+ plate[' A2' ],
772
+ blow_out = True , # blow out droplets into the source well (A1 of "plate")
773
+ new_tip = ' never' )
774
+
775
+ .. versionadded :: 2.8
776
+
777
+
778
+ ``blowout_location ``
779
+ --------------------
780
+
781
+ Starting in Python API Version 2.8 and above, you can specify the well type you would like your pipette to blow out in.
782
+ Specifying a location will override any defaults from the ``blow_out `` argument.
783
+
784
+ The code below is the same default behavior you would see utilizing ``blow_out=True `` only. It will blow out for
785
+ every transfer into the trash.
786
+
787
+ .. code-block :: python
788
+
789
+ pipette.transfer(
790
+ 100 ,
791
+ plate[' A1' ],
792
+ plate[' A2' ],
793
+ blow_out = True ,
794
+ blowout_location = ' trash' ) # blow out droplets into the trash
795
+
796
+ .. versionadded :: 2.8
797
+
798
+ The same is true even if you have extra liquid left in your tip shown below.
799
+
800
+ .. code-block :: python
801
+
802
+ pipette.pick_up_tip()
803
+ pipette.aspirate(10 , plate[' A1' ])
804
+ pipette.transfer(
805
+ 100 ,
806
+ plate[' A1' ],
807
+ plate[' A2' ],
808
+ blow_out = True ,
809
+ blowout_location = ' trash' , # blow out droplets into the trash
810
+ new_tip = ' never' )
811
+
812
+ .. versionadded :: 2.8
813
+
814
+ If you wish to blow out in the source or destination well you can do so by specifying the location as either ``source well `` or ``destination well ``.
815
+ For example, to blow out in the destination well you can do the following:
816
+
817
+ .. code-block :: python
818
+
819
+ pipette.transfer(
820
+ 100 ,
821
+ plate[' A1' ],
822
+ plate.wells(),
823
+ blow_out = True ,
824
+ blowout_location = ' destination well' ) # blow out droplets into each destination well (this will blow out in wells `A1`, `B1`, `C1`..etc)
825
+
826
+ .. versionadded :: 2.8
827
+
828
+
829
+ .. note ::
830
+
831
+ You *must * specify ``blow_out=True `` in order to utilize the new argument ``blowout_location ``
832
+
833
+
834
+
754
835
``mix_before ``, ``mix_after ``
755
836
-----------------------------
756
837
0 commit comments