|
| 1 | +export const reagentTransfer = ` |
| 2 | +Write a protocol for the Opentrons OT-2 as described below: |
| 3 | +
|
| 4 | +Metadata: |
| 5 | +- Application: Reagent transfer |
| 6 | +- Robot: OT-2 |
| 7 | +- API: 2.15 |
| 8 | +
|
| 9 | +Pipette mount: |
| 10 | +- P1000 Single-Channel GEN2 is mounted on left |
| 11 | +- P300 Single-Channel GEN2 is mounted on right |
| 12 | +
|
| 13 | +Labware: |
| 14 | +- Source Labware: Thermo Scientific Nunc 96 Well Plate 2000 µL on slot 7 |
| 15 | +- Destination Labware: Opentrons 24 Well Aluminum Block with NEST 0.5 mL Screwcap on slot 3 |
| 16 | +- Tiprack: Opentrons 96 Filter Tip Rack 1000 µL on slot 4 |
| 17 | +
|
| 18 | +Commands: |
| 19 | +- Using P1000 Single-Channel GEN2 pipette on left mount, transfer 195.0 uL of reagent |
| 20 | + from H10, F12, D7, B1, C8 wells in source labware |
| 21 | + to first well in the destination labware. |
| 22 | + Use new tip for each transfer. |
| 23 | +` |
| 24 | + |
| 25 | +export const flexReagentTransfer = ` |
| 26 | +Write a protocol for the Opentrons Flex as described below: |
| 27 | +
|
| 28 | +Metadata and requirements: |
| 29 | +- Application: Reagent transfer |
| 30 | +- Robot: Flex |
| 31 | +- API: 2.15 |
| 32 | +
|
| 33 | +Pipette Mount: |
| 34 | +- Flex 1-Channel 1000 µL Pipette is mounted on the left side |
| 35 | +- Flex 1-Channel 50 µL Pipette is mounted on the right side |
| 36 | +
|
| 37 | +Labware: |
| 38 | +- Source Labware 1: NEST 1 Well Reservoir 195 mL is positioned on slot B1 |
| 39 | +- Source Labware 2: Bio-Rad 384 Well Plate 50 µL is positioned on slot B2 |
| 40 | +- Source Labware 3: Bio-Rad 96 Well Plate 200 µL is positioned on slot B3 |
| 41 | +- Destination Labware 1: Corning 384 Well Plate 112 µL Flat is positioned on slot D1 |
| 42 | +- Destination Labware 2: Corning 96 Well Plate 360 µL Flat is positioned on slot D2 |
| 43 | +- Tiprack 1: Opentrons Flex 96 Filter Tip Rack 200 µL is used on slot A1 |
| 44 | +- Tiprack 2: Opentrons Flex 96 Filter Tip Rack 50 µL is used on slot A2 |
| 45 | +
|
| 46 | +Commands |
| 47 | +- Using Flex 1-Channel 50 µL Pipette on right mount, transfer 15 µL from first of source labware 1 to each well |
| 48 | + in destination labware 1 and destination labware 2. Reuse the same tip. |
| 49 | +- Again using Flex 1-Channel 50 µL Pipette, transfer 20 µL from each well in source labware 2 to |
| 50 | + each well in the destination labware 1. Reuse the same tip. |
| 51 | +- Using Flex 1-Channel 1000 µL Pipette on left mount, transfer 100µL liquid from each well in source labware 3 |
| 52 | + to each well in destination labware 2. Use a new tip each time. |
| 53 | +` |
| 54 | + |
| 55 | +export const pcr = ` |
| 56 | +Write a protocol for the Opentrons OT-2 as described below: |
| 57 | +
|
| 58 | +Metadata: |
| 59 | +- Application: ThermoPrime Taq DNA Polymerase, with 10x buffer and separate vial of 25 mM MgCl2Thermo Scientific kit PCR amplification |
| 60 | +- Robot: OT-2 |
| 61 | +- API: 2.15 |
| 62 | +
|
| 63 | +Pipette mount: |
| 64 | +- P20 Single Channel is mounted on the right side |
| 65 | +
|
| 66 | +Modules: |
| 67 | +- Thermocycler module is present on slot 7 |
| 68 | +- Temperature module is place on slot 3 |
| 69 | +
|
| 70 | +Labware: |
| 71 | +- Source sample labware is Opentrons 96 Well Aluminum Block with NEST Well Plate 100 µL plate placed on slot 1 |
| 72 | +- Source mastermix labware is Opentrons 24 Well Aluminum Block with NEST 1.5 mL Snapcap, placed on temperature module on slot 3 |
| 73 | +- Destination Labware is an Opentrons Tough 96 Well Plate 200 µL PCR Full Skirt placed on thermocycler module on slot 7 |
| 74 | +- 20 ul Filter tiprack is used on slot 4 |
| 75 | +
|
| 76 | +Well allocation: |
| 77 | +- source wells are first 41 wells column wise in both master mix and sample source plates |
| 78 | +- destination wells: first 41 wells column wise on thermocycler |
| 79 | +
|
| 80 | +Commands: |
| 81 | +Note that every step is a single entity. Do not combine. Also, every step should be performed in order. |
| 82 | +1. The total number of samples is 41 |
| 83 | +2. Set the thermocycler such that: |
| 84 | + - block temperature is 6 degree C |
| 85 | + - lid temperature to 90 degree C |
| 86 | + - lid open |
| 87 | +3. Set the master mix temperature module at 10 C. The temperature module wait time is 50 seconds. |
| 88 | +4. Transfer 10 uL of mastermix from source well to destination well. Use the same pipette tip for all transfers. |
| 89 | +5. Transfer 3 ul of sample to destination well reusing tip everytime. After dispensing, mix the sample and mastermix |
| 90 | +of 13 ul total volume 4 times and then perform blowout before dropping tip. |
| 91 | +6. Close the lid of the thermocycler. |
| 92 | +7. Set the thermocycle to following parameters (**note that each step is independent**): |
| 93 | + Step 1: 66 degree C for 47 seconds for 1 cycles |
| 94 | + Step 2: 88 degree C for 28 seconds, 82 degree C for 14 seconds, 68 degree C for 68 seconds for 15 cycles |
| 95 | + Step 3: 70 degree C for 240 seconds for 1 cycles |
| 96 | +Then, execute thermocycler profile for each step. |
| 97 | +8. After the above three steps are completed, hold thermocycler block at 4 C |
| 98 | +9. Open thermocycler lid |
| 99 | +10. Deactivate the temperature modules |
| 100 | +` |
| 101 | + |
| 102 | +export const flexPcr = ` |
| 103 | +Write a protocol for the Opentrons Flex as described below: |
| 104 | +
|
| 105 | +Metadata and requirements: |
| 106 | +- Application: GeneAmp2x PCR amplification |
| 107 | +- Robot: Flex |
| 108 | +- API: 2.15 |
| 109 | +
|
| 110 | +Pipette mount: |
| 111 | +- Flex 1-Channel 50 µL Pipette is mounted on the right side |
| 112 | +
|
| 113 | +Modules and adapters: |
| 114 | +- Thermocycler GEN 2 module is present on slot A1+B1 |
| 115 | +- Temperature module GEN 2 is place on slot D3 |
| 116 | +
|
| 117 | +Labware: |
| 118 | +- Source sample labware is Opentrons 96 Well Aluminum Block with NEST Well Plate 100 µL plate placed on slot D1 |
| 119 | +- Source mastermix labware is Opentrons 24 Well Aluminum Block with NEST 1.5 mL Snapcap, placed on temperature module on slot D3 |
| 120 | +- Destination Labware is an Opentrons Tough 96 Well Plate 200 µL PCR Full Skirt placed on thermocycler GEN 2 module |
| 121 | +- Opentrons Flex 96 Filter Tip Rack 50 µL is used on slot C1 |
| 122 | +
|
| 123 | +Sample position: |
| 124 | +- source wells are first 64 wells column wise in both master mix and sample source plates |
| 125 | +- destination wells: first 64 wells column wise on thermocycler |
| 126 | +
|
| 127 | +Commands: |
| 128 | +Note that every step is a single entity. Do not combine. Also, every step should be performed in order. |
| 129 | +1. The total number of samples is 64 |
| 130 | +2. Set the thermocycler such that |
| 131 | + - block temperature is 6 degree C |
| 132 | + - lid temperature to 90 degree C |
| 133 | + - lid open |
| 134 | +3. Set the master mix temperature module at 10 C. The temperature module wait time is 50 seconds. |
| 135 | +4. Transfer 10 uL of mastermix from source well to destination well. Use the same pipette tip for all transfers. |
| 136 | +5. Transfer 3 ul of sample to destination well reusing tip everytime. After dispensing, mix the sample and mastermix |
| 137 | +of 13 ul total volume 4 times and then perform blowout before dropping tip. |
| 138 | +6. Close the lid of the thermocycler. |
| 139 | +7. Set the thermocycle to following parameters (**note that each step is independent**): |
| 140 | + Step 1: 66 degree C for 47 seconds for 1 cycles |
| 141 | + Step 2: 88 degree C for 28 seconds, 82 degree C for 14 seconds, 68 degree C for 68 seconds for 15 cycles |
| 142 | + Step 3: 70 degree C for 240 seconds for 1 cycles |
| 143 | +Then, execute thermocycler profile for each step. |
| 144 | +8. After the above three steps are completed, hold thermocycler block at 4 C |
| 145 | +9. Open thermocycler lid |
| 146 | +10. Deactivate the temperature modules |
| 147 | +` |
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