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* Aspirate 100 uL of liquid from well A1 on your plate
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* Take p200 pipette
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* Aspirate 100 uL from well A1 on your plate
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* Dispense everything into well A2 on the same plate
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**Permissive**: everyone's process is different and we are not trying to impose our way of thinking on you. Instead, our API allows for different ways of expressing your protocol and adding fine details as you need them.
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p200.aspirate(100, plate[0]).dispense(plate[1])
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while using 0 or 1 instead of 'A1' and 'A2' will do just the same.
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while using 0 or 1 instead of 'A1' and 'B1' will do just the same.
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or
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@@ -132,6 +86,39 @@ or
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will aspirate 100, from the bottom of a well.
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Hello World
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-----------
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Below is a short protocol that will pick up a tip and use it to move 100ul volume across all the wells on a plate:
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.. code-block:: python
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from opentrons import robot
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from opentrons import containers, instruments
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tiprack = containers.load(
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'tiprack-200ul', # container type
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'A1', # slot
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'tiprack'# user-defined name
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)
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plate = containers.load('96-flat', 'B1', 'plate')
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p200 = instruments.Pipette(
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axis="b",
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max_volume=200
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)
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p200.pick_up_tip(tiprack[0])
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for i inrange(95):
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p200.aspirate(100, plate[i])
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p200.dispense(plate[i +1])
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p200.return_tip()
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robot.simulate()
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Installing Opentrons API
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------------------------
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If you are just starting with Python it is recommended to install Jupyter notebook to run Opentrons API. Please refer to `Setting up Opentrons API`_ for detailed instructions.
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.. code-block:: bash
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pip install opentrons
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What's next?
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---------------
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* Start with `Setting up Opentrons API`_ in `Jupyter <http://jupyter.org/>`_.
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* Write your first `Hello World API Protocol`_.
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* Learn some common `Tips and Tricks for API Liquid Handling`_.
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* Discover `Full API Documentation`_ for advanced API protocol writing.
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