|
2 | 2 | PEtab Tutorial |
3 | 3 | ============== |
4 | 4 |
|
| 5 | +.. _Boehm et al. (2014): https://dx.doi.org/10.1021/pr5006923 |
| 6 | + |
| 7 | + |
5 | 8 | Overview |
6 | 9 | ++++++++ |
7 | 10 |
|
8 | 11 | In the following, we demonstrate how to set up a parameter estimation |
9 | 12 | problem in PEtab based on a realistic application example. To this end, |
10 | 13 | we consider the model and experimental data by |
11 | | -`Boehm et al. (2014) <https://pubs.acs.org/doi/abs/10.1021/pr5006923>`_. The |
| 14 | +`Boehm et al. (2014)`_. The |
12 | 15 | model describes the dynamics of phosphorylation and dimerization of the |
13 | 16 | transcription factors STAT5A and STAT5B. A visualization and the |
14 | 17 | corresponding reactions of the model are provided below, although the |
15 | 18 | details of the model are not relevant for the purpose of this tutorial. |
16 | | -For more details, we refer to the original publication (Boehm et al., |
17 | | -2014). |
| 19 | +For more details, we refer to the original publication. |
18 | 20 |
|
19 | 21 | A PEtab problem consists of 1) an SBML model of a biological system, 2) |
20 | 22 | condition, observable and measurement definitions, and 3) the |
@@ -58,7 +60,7 @@ tutorial is available `online <https://github.com/PEtab-dev/PEtab/tree/b50d000a5 |
58 | 60 | 2. Linking model and measurements |
59 | 61 | +++++++++++++++++++++++++++++++++ |
60 | 62 |
|
61 | | -The model by Boehm et al. (2014) was calibrated on measurements on |
| 63 | +The model by `Boehm et al. (2014)`_ was calibrated on measurements on |
62 | 64 | phosphorylation levels of STAT5A and STAT5B as well as relative STAT5A |
63 | 65 | abundance for different timepoints between 0 - 240 minutes after |
64 | 66 | stimulation with erythropoietin (Epo): |
@@ -242,7 +244,7 @@ PEtab measurement file: |
242 | 244 | 3. Defining parameters |
243 | 245 | ++++++++++++++++++++++ |
244 | 246 |
|
245 | | -The model by Boehm et al. (2014) contains nine unknown parameters that |
| 247 | +The model by `Boehm et al. (2014)`_ contains nine unknown parameters that |
246 | 248 | need to be estimated from the experimental data. Additionally, it has |
247 | 249 | two known parameters that are fixed to literature values. |
248 | 250 |
|
@@ -414,3 +416,12 @@ be found at the PEtab Github page |
414 | 416 | .. [#f1] |
415 | 417 | TSV files can be created using any standard spreadsheet application, |
416 | 418 | or for small files, text editor. |
| 419 | +
|
| 420 | +
|
| 421 | +References |
| 422 | +++++++++++ |
| 423 | + |
| 424 | +* Martin E. Boehm, Lorenz Adlung, Marcel Schilling, Susanne Roth, |
| 425 | + Ursula Klingmüller, and Wolf D. Lehmann. |
| 426 | + *Journal of Proteome Research* **2014** 13 (12), 5685-5694. |
| 427 | + DOI: `10.1021/pr5006923 <https://dx.doi.org/10.1021/pr5006923>`_. |
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