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experiments/02-Secondary level education/salt-stress-on-yeast.md

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:::tip Success Goal
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Use your Pioreactor to model how cells are affected by high salt (hypertonic) solutions.
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2020
## Requirements
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We performed this experiment using five Pioreactors and vials containing 15 mL of sterile YPD stock and salt concentrations of 0%, 1.5%, 3%, 4.5%, and 6% w/v.
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A sample calculation is as follows:
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$$
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\text \{w/v \%\}= \frac \{\text\{mass\}\}\{\text \{volume\}\}
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\text{w/v \%} = \frac{\text{mass}}{\text{volume}}
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$$
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To create a salt concentration of **3% w/v** for **15 mL** of media:
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$$
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0.03 = \frac \{\text\{mass\}\}\{15 mL\}
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0.03 = \frac{\text{mass}}{15 \text{ mL}}
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$$
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$$
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\text \{mass\} = 0.45 g
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\text{mass} = 0.45 \text{ g}
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$$
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Therefore, **0.45 grams** of table salt is needed for 3% w/v of salt.
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To each vial, 0g, 0.225g, 0.45g, 0.675g and 0.9g of table salt were added, repectively. They were then innoculated with one drop of rehydrated active yeast (0.500 g of dry yeast in a 15 mL YPD broth vial). The _Temperature automation_ was set on _Thermostat_ at 32.5°C.

experiments/02-Secondary level education/yeast-growth-by-temperature.md

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:::tip Success Goal
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Demonstrate the effect of temperature on yeast growth.
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## Requirements
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