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collapsed: true
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collapsible: true
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label: "Pioreactor 40ml"
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label: "Pioreactor 40ml v1.0"

user-guide/03-Extending your Pioreactor/05-calibrate-od600.md

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To begin calibrations, type `pio calibrations run --device od`, and choose the protocol. Start off by inputting some metadata of your calibration. Provide a descriptive and unique name to make it easier to find your calibration again, if needed. Follow the rest of the questions.
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:::info
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One of your photodiodes will be located in the 90° or 135° pocket on the vial holder. You will be prompted to confirm the angle of your PD cable. If this is incorrect, go to the _Configuration_ tab on the Pioreactor UI and under _od_config.photodiode_channel_, change to your angle of choice, then restart your OD calibration.
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![Change the angle through the UI configuration tab.](/img/user-guide/change_angle.png)
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:::
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Following the questions, stirring and optical density reading will begin. A graph is generated as you calibrate so you can see your progress.
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user-guide/03-Extending your Pioreactor/08-hardware-calibrations.md

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Hardware calibrations serve as a method to get accurate performance from your Pioreactor.
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### Stirring calibration (optional)
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### Stirring calibrations
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:::info
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You'll need the following:
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1. Pioreactor HAT
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2. Pioreactor sleeve
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3. Vial and stirbar
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:::
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Internally, the Pioreactor will vary the amount of voltage to apply to the stirring motor to reach the desired RPM, however, this can take a few minutes. We can significantly improve this by asking the Pioreactor to create a "map" between voltage and RPM beforehand. Then the Pioreactor only needs to perform a lookup in the map to hit the desired RPM.
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:::note
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:::tip
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This is optional, but can really help stirring performance. Creating a stirring calibration is also very easy, so we recommend it.
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:::
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<AssemblyInstructionBlock title="Calibrating the stirring in the UI" images={["user-guide/calibrate.png","user-guide/calibrate_stirring.png"]}>
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1. Start by filling a Pioreactor vial about 3/4th with water, and place the stirbar inside. Close with lid. Place into the Pioreactor.
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1. We'll perform this routine through your computer's command line. Need help [accessing it](/user-guide/accessing-raspberry-pi)?
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2. Enter **`ssh pioreactor@<insert unit name>.local`**.
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4. The Pioreactor will increment the voltage applied to the motor, and record the RPM. After it's done, it will collect this data into a calibration curve, and store it locally.
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4. At the end, you'll be prompted to set this as the "active" calibration. Select "Yes".
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</AssemblyInstructionBlock>
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You're done! Your stirring RPM should be much more responsive now.
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## Managing calibrations
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From both the UI and the command line, you can manage your Pioreactor's calibrations.
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![Calibrations page showing filters, table, and chart.](/img/user-guide/calibrations-page.png)
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**In the UI**
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- Use the `Pioreactor` and `Device` filters at the top of the Calibrations page to focus on a specific unit and hardware type. Toggle **Only Active calibrations** to hide everything except the curve currently in use.
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- Click any calibration row to open its detail view. Use **Set active** to mark that calibration as the one the Pioreactor will use for that device (only one calibration can be active per device per Pioreactor). Use **Set inactive** to clear it and disable calibration for that device.
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- The chart in both the list view and detail view has a download icon—click it to export a PNG of the calibration curve for lab notes. The top-level **Download all calibrations** button grabs all calibration YAML files for backup.
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- To edit a calibration, open its detail view and choose **View YAML**. Make small changes, save the YAML, and re-upload it via **Upload calibration** (or edit the YAML on-disk and use `pio calibrations display` to confirm before setting it active).
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**From the command line**
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- List what you have with `pio calibrations list` or scope to a single device with `pio calibrations list --device <device>`.
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- Set which calibration is active with `pio calibrations set-active --device <device> --name <calibration_name>`. Omit `--name` to clear the active calibration for that device.

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