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lines changed Original file line number Diff line number Diff line change @@ -48,12 +48,22 @@ This takes less than 4 seconds to load via a network drive on my machine.
4848
4949 sudo pip install pyreference
5050
51- Pre-process your GFF3 or GTF files to create genes.gtf.json.gz (~ 1/20th the size of the input GTF file)
51+ Choose your annotation:
52+
53+ # Latest Ensembl GRCh37
54+ wget ftp://ftp.ensembl.org/pub/grch37/release-87/gff3/homo_sapiens/Homo_sapiens.GRCh37.87.gff3.gz
55+
56+ # Latest Ensembl GRCh38
57+ wget ftp://ftp.ensembl.org/pub/release-104/gff3/homo_sapiens/Homo_sapiens.GRCh38.104.gff3.gz
5258
53- pyreference_gff_to_json.py --gtf genes.gtf
59+ # Latest RefSeq GRCh37
60+ wget http://ftp.ncbi.nlm.nih.gov/refseq/H_sapiens/annotation/annotation_releases/105.20201022/GCF_000001405.25_GRCh37.p13/GCF_000001405.25_GRCh37.p13_genomic.gff.gz
5461
55- or
62+ # Latest RefSeq GRCh38
63+ http://ftp.ncbi.nlm.nih.gov/refseq/H_sapiens/annotation/annotation_releases/109.20210514/GCF_000001405.39_GRCh38.p13/GCF_000001405.39_GRCh38.p13_genomic.gff.gz
5664
65+ Pre-process your GFF3 or GTF files to create genes.gtf.json.gz (~ 1/20th the size of the input GTF file)
66+
5767 pyreference_gff_to_json.py --gff3 genes.gff.gz
5868
5969Create a ~ /pyreference.cfg file pointing to your references.
@@ -78,7 +88,6 @@ Substitute ArgumentParser with pyreference.ReferenceArgumentParser to add a --bu
7888
7989args.reference is now initialised to the correct build/annotation.
8090
81-
8291 from pyreference import ReferenceArgumentParser
8392
8493 parser = ReferenceArgumentParser()
Original file line number Diff line number Diff line change 44setup (name = 'pyreference' ,
55 packages = find_packages (),
66 version = '0.6' ,
7- description = 'Library for working with reference genomes' ,
7+ description = 'Library for working with reference genomes and gene GTF/GFFs ' ,
88 author = 'David Lawrence' ,
99 author_email = 'davmlaw@gmail.com' ,
1010 url = 'https://github.com/SACGF/pyreference' ,
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