diff --git a/README.md b/README.md
index 9952638d1..673431a54 100644
--- a/README.md
+++ b/README.md
@@ -233,7 +233,7 @@ To learn more about the docker pull rate limits and the open source software pro
| [metaphlan](https://hub.docker.com/r/staphb/metaphlan)
[](https://hub.docker.com/r/staphb/metaphlan) | Click to see all versions
- [3.0.3-no-db (no database)](./build-files/metaphlan/3.0.3-no-db/)
- [3.0.3 (~3GB db)](./build-files/metaphlan/3.0.3/)
- [4.1.0](./build-files/metaphlan/4.1.0/) (no database)
- [4.1.1](./build-files/metaphlan/4.1.1/) (no database)
- [4.2.2](./build-files/metaphlan/4.2.2/)
- [4.2.4](./build-files/metaphlan/4.2.4/)
| https://github.com/biobakery/MetaPhlAn |
| [MIDAS](https://hub.docker.com/r/staphb/midas)
[](https://hub.docker.com/r/staphb/midas) | Click to see all versions
- [1.3.2 (no database)](./build-files/midas/1.3.2/)
| https://github.com/snayfach/MIDAS |
| [minimap2](https://hub.docker.com/r/staphb/minimap2)
[](https://hub.docker.com/r/staphb/minimap2) | Click to see all versions
- [2.17](./build-files/minimap2/2.17/)
- [2.18](./build-files/minimap2/2.18/)
- [2.21](./build-files/minimap2/2.21/)
- [2.22](./build-files/minimap2/2.22/)
- [2.23](./build-files/minimap2/2.23/)
- [2.24](./build-files/minimap2/2.24/)
- [2.25](./build-files/minimap2/2.25/)
- [2.26](./build-files/minimap2/2.26)
- [2.27](./build-files/minimap2/2.27/)
- [2.28](./build-files/minimap2/2.28/)
- [2.29](./build-files/minimap2/2.29/)
- [2.30](./build-files/minimap2/2.30/)
| https://github.com/lh3/minimap2 |
-| [minipolish](https://hub.docker.com/r/staphb/minipolish)
[](https://hub.docker.com/r/staphb/minipolish) | Click to see all versions
- [0.1.3](./build-files/minipolish/0.1.3/)
- [0.2.0](./build-files/minipolish/0.2.0/)
| https://github.com/rrwick/Minipolish |
+| [minipolish](https://hub.docker.com/r/staphb/minipolish)
[](https://hub.docker.com/r/staphb/minipolish) | Click to see all versions
- [0.1.3](./build-files/minipolish/0.1.3/)
- [0.2.0](./build-files/minipolish/0.2.0/)
- [0.2.1](./build-files/minipolish/0.2.1/)
| https://github.com/rrwick/Minipolish |
| [mlst](https://hub.docker.com/r/staphb/mlst)
[](https://hub.docker.com/r/staphb/mlst) | Click to see all versions
- [2.16.2](./build-files/mlst/2.16.2/)
- [2.17.6](./build-files/mlst/2.17.6/)
- [2.19.0](./build-files/mlst/2.19.0/)
- [2.22.0](./build-files/mlst/2.22.0/)
- [2.22.1](./build-files/mlst/2.22.1/)
- [2.23.0](./build-files/mlst/2.23.0/)
- [2.23.0-2023-07](./build-files/mlst/2.23.0-2023-07/)
- [2.23.0-2023-08](./build-files/mlst/2.23.0-2023-08/)
- [2.23.0-2024-01](./build-files/mlst/2.23.0-2024-01/)
- [2.23.0-2024-03](./build-files/mlst/2.23.0-2024-12/)
- [2.23.0-2024-12-31](./build-files/mlst/2.23.0-2024-12-31/)
- [2.25.0](./build-files/mlst/2.25.0/)
- [2.28.1](./build-files/mlst/2.28.1/)
- [2.32.2](./build-files/mlst/2.32.2/)
| https://github.com/tseemann/mlst |
| [MMseqs2](https://hub.docker.com/r/staphb/mmseqs2)
[](https://hub.docker.com/r/staphb/mmseqs2) | Click to see all versions
- [17-b804f](./build-files/mmseqs2/17-b804f/)
- [18-8cc5c](./build-files/mmseqs2/18-8cc5c/)
| https://github.com/soedinglab/MMseqs2 |
| [mob-suite](https://github.com/phac-nml/mob-suite)
[](https://hub.docker.com/r/staphb/mob-suite) | Click to see all versions
- [3.1.9](./build-files/mob-suite/3.1.9/)
| https://github.com/phac-nml/mob-suite |
diff --git a/build-files/minipolish/0.2.1/Dockerfile b/build-files/minipolish/0.2.1/Dockerfile
new file mode 100644
index 000000000..ca1551d63
--- /dev/null
+++ b/build-files/minipolish/0.2.1/Dockerfile
@@ -0,0 +1,116 @@
+ARG MINIPOLISH_VER=0.2.1
+ARG MINIMAP2_VER=2.30
+ARG RACON_VER=1.5.0
+ARG MINIASM_VER=0.3
+
+FROM ubuntu:noble AS builder
+ARG MINIPOLISH_VER
+ARG MINIMAP2_VER
+ARG RACON_VER
+ARG MINIASM_VER
+
+# Install dependencies
+RUN apt-get update && apt-get install --no-install-recommends -y \
+ perl \
+ default-jre \
+ gnuplot \
+ libgomp1 \
+ maven \
+ git \
+ wget \
+ python3 \
+ build-essential \
+ cmake \
+ zlib1g-dev \
+ curl \
+ bzip2 && \
+ apt-get autoclean && rm -rf /var/lib/apt/lists/*
+
+# Install Racon
+RUN wget https://github.com/lbcb-sci/racon/archive/refs/tags/${RACON_VER}.tar.gz && \
+ tar -xvf ${RACON_VER}.tar.gz && \
+ cd racon-${RACON_VER} && \
+ mkdir build && \
+ cd build && \
+ cmake -DCMAKE_BUILD_TYPE=Release .. && \
+ make
+
+# Add Racon to PATH
+ENV PATH="/racon-${RACON_VER}/build/bin:${PATH}"
+
+# Test Racon
+RUN racon_test
+
+# Install Minimap2
+RUN curl -L https://github.com/lh3/minimap2/releases/download/v${MINIMAP2_VER}/minimap2-${MINIMAP2_VER}_x64-linux.tar.bz2 | tar -jxvf - --no-same-owner && \
+ mv minimap2-${MINIMAP2_VER}_x64-linux /usr/local/bin/minimap2
+
+# miniasm
+RUN wget https://github.com/lh3/miniasm/archive/v${MINIASM_VER}.tar.gz && \
+ mkdir miniasm && \
+ tar -xzvf v${MINIASM_VER}.tar.gz -C miniasm --strip-components 1 && \
+ rm v${MINIASM_VER}.tar.gz && \
+ cd miniasm && \
+ make && \
+ mv miniasm /usr/local/bin/.
+
+FROM ubuntu:noble AS app
+ARG MINIPOLISH_VER
+ARG RACON_VER
+
+# metadata
+LABEL base.image="ubuntu:noble"
+LABEL dockerfile.version="1"
+LABEL software="minipolish"
+LABEL software.version="${MINIPOLISH_VER}"
+LABEL description="Racon polishing for GFA graphs produced by miniasm"
+LABEL website="https://github.com/rrwick/Minipolish"
+LABEL license="https://github.com/rrwick/Minipolish/blob/main/LICENSE"
+LABEL maintainer="Erin Young"
+LABEL maintainer.email="eriny@utah.gov"
+
+# install dependencies via apt-get or yum if using a centos or fedora base
+RUN apt-get update && apt-get install -y --no-install-recommends \
+ wget \
+ ca-certificates \
+ python3-pip && \
+ apt-get autoclean && rm -rf /var/lib/apt/lists/*
+
+COPY --from=builder /racon-${RACON_VER}/build/bin/* /usr/local/bin/
+COPY --from=builder /usr/local/bin/* /usr/local/bin/
+
+# install minipolish
+RUN wget https://github.com/rrwick/Minipolish/archive/refs/tags/v${MINIPOLISH_VER}.tar.gz && \
+ tar -vxf v${MINIPOLISH_VER}.tar.gz && \
+ pip install --break-system-packages v${MINIPOLISH_VER}.tar.gz && \
+ rm v${MINIPOLISH_VER}.tar.gz
+# in theory, this can be tested with python3 -m pytest
+
+# set environmental variables e.g. $PATH and locale settings for singularity compatibility
+ENV PATH="/Minipolish-${MINIPOLISH_VER}:${PATH}" \
+ TERM=xterm-256color \
+ LC_ALL=C
+
+# set working directory
+WORKDIR /data
+
+CMD ["minipolish", "--help"]
+
+FROM app AS test
+
+RUN minipolish --help
+
+# checking dependencies
+RUN minimap2 --version && racon --help && miniasm -V
+
+WORKDIR /test
+
+RUN wget https://github.com/rrwick/Unicycler/raw/refs/heads/main/sample_data/long_reads_high_depth.fastq.gz
+
+#RUN miniasm_and_minipolish.sh long_reads_high_depth.fastq.gz 4 > test.gfa && head test.gfa
+
+# Testing the test data without using the wrapper script
+RUN minimap2 -x ava-ont -t 4 long_reads_high_depth.fastq.gz long_reads_high_depth.fastq.gz > overlaps.paf && \
+ miniasm -f long_reads_high_depth.fastq.gz overlaps.paf -s 50 -e 1 > test.gfa && \
+ minipolish --threads 4 long_reads_high_depth.fastq.gz test.gfa > polished.gfa && \
+ head polished.gfa
diff --git a/build-files/minipolish/0.2.1/README.md b/build-files/minipolish/0.2.1/README.md
new file mode 100644
index 000000000..0bd527e00
--- /dev/null
+++ b/build-files/minipolish/0.2.1/README.md
@@ -0,0 +1,22 @@
+# minipolish and miniasm container
+
+Main tool : [minipolish](https://github.com/rrwick/Minipolish) and [miniasm](https://github.com/lh3/miniasm)
+
+Other tools :
+- [racon](https://github.com/isovic/racon) version 1.5.0
+- [minimap2](https://github.com/lh3/minimap2) version 2.30
+- [miniasm](https://github.com/lh3/miniasm) version 0.3
+
+From minipolish's [readme](https://github.com/rrwick/Minipolish):
+
+> Miniasm is a great long-read assembly tool: straight-forward, effective and very fast. However, it does not include a polishing step, so its assemblies have a high error rate – they are essentially made of stitched-together pieces of long reads.
+>
+> Racon is a great polishing tool that can be used to clean up assembly errors. It's also very fast and well suited for long-read data. However, it operates on FASTA files, not the GFA graphs that miniasm makes.
+>
+> That's where Minipolish comes in. With a single command, it will use Racon to polish up a miniasm assembly, while keeping the assembly in graph form.
+
+# Example Usage
+
+```
+miniasm_and_minipolish.sh input.fastq $threads > output.gfa
+```