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lines changed Original file line number Diff line number Diff line change @@ -49,14 +49,15 @@ to run 4Pipe4. If you are using linux you can get python 3 from you
4949distribution's package manager (sudo apt-get install python3 for Ubuntu) or get
5050it from the website (http://python.org/download/ ). Also required are the python3
5151header files (the package name in Ubuntu is python3-dev).
52+ Due to the latest release of pysam, python >= 3.4 is now required.
5253Not strictly required, but highly recommended to for best results are the
5354external programs that 4Pipe4 uses in it's processes. By default, these are:
5455
5556* seqclean (http://compbio.dfci.harvard.edu/tgi/software/ )
5657* mira 4.x series (http://mira-assembler.sourceforge.net/ )
5758* getorf (http://emboss.sourceforge.net/apps/cvs/emboss/apps/getorf.html )
5859* blast (ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/)
59- * blast2go4pipe (http://www.blast2go.com/b2glaunch/resources )
60+ * blast2go4pipe (http://www.blast2go.com/b2glaunch/resources ) - * Seems to have been discontinued *
6061* etandem (http://helixweb.nih.gov/emboss/html/etandem.html )
6162* 7zip (http://www.7-zip.org/ )
6263* pysam (https://github.com/pysam-developers/pysam )
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