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38 | 38 | colorfiles{3} = [stem0312 'ebfp2_P3.fcs']; |
39 | 39 |
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40 | 40 | % FSC and SSC channels can be added to be read unprocessed to MEFL |
41 | | -channels{4} = Channel('FSC-A', 488, 488, 10); |
42 | | -channels{4} = setPrintName(channels{4}, 'FSC'); |
43 | | -channels{4} = setLineSpec(channels{4}, 'k'); |
| 41 | +% channels{4} = Channel('FSC-A', 488, 488, 10); |
| 42 | +% channels{4} = setPrintName(channels{4}, 'FSC'); |
| 43 | +% channels{4} = setLineSpec(channels{4}, 'k'); |
44 | 44 | % If the name is FSC or SSC (or one of those with '-A', '-H', or '-W') it will automatically be unprocessed; otherwise, set it |
45 | 45 | % channels{4} = setIsUnprocessed(channels{4}, true); |
46 | 46 |
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56 | 56 |
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57 | 57 | % Size bead files are used for processing FSC-A units into um equivalent diameter |
58 | 58 | % They are optional, and will not be used if size bead file is not set |
59 | | -% sizebeadfile = []; |
60 | | -sizebeadfile = '../example_controls/180614_PPS6K_A02.fcs'; |
| 59 | +sizebeadfile = []; |
| 60 | +% sizebeadfile = '../example_controls/180614_PPS6K_A02.fcs'; |
61 | 61 |
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62 | 62 | CM = ColorModel(beadfile, blankfile, channels, colorfiles, colorpairfiles, sizebeadfile); |
63 | 63 | CM=set_translation_plot(CM, true); |
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79 | 79 | CM=set_translation_channel_min(CM,[2,2,2]); |
80 | 80 |
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81 | 81 | % Configuration for size beads, if used |
82 | | -TASBEConfig.set('sizebeads.beadModel','SpheroTech PPS-6K'); % Entry from BeadCatalog.xls matching your beads |
| 82 | +% TASBEConfig.set('sizebeads.beadModel','SpheroTech PPS-6K'); % Entry from BeadCatalog.xls matching your beads |
83 | 83 | % Can also set bead channel or batch, if alternatives are available |
84 | 84 | % Ignore all size bead data below 10^[rangeMin] as being too "smeared" with noise |
85 | | -TASBEConfig.set('sizebeads.rangeMin', 2); |
86 | | -CM=set_um_channel_name(CM, 'FSC-A'); |
| 85 | +% TASBEConfig.set('sizebeads.rangeMin', 2); |
| 86 | +% CM=set_um_channel_name(CM, 'FSC-A'); |
87 | 87 |
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88 | 88 | % When dealing with very strong fluorescence, use secondary channel to segment |
89 | 89 | % TASBEConfig.set('beads.secondaryBeadChannel','PE-Tx-Red-YG-A'); |
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