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jakebeal
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ready for testing with size bead enhancement
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template_colormodel/CM120312.mat

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template_colormodel/make_color_model.m

Lines changed: 8 additions & 8 deletions
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@@ -38,9 +38,9 @@
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colorfiles{3} = [stem0312 'ebfp2_P3.fcs'];
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% FSC and SSC channels can be added to be read unprocessed to MEFL
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channels{4} = Channel('FSC-A', 488, 488, 10);
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channels{4} = setPrintName(channels{4}, 'FSC');
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channels{4} = setLineSpec(channels{4}, 'k');
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% channels{4} = Channel('FSC-A', 488, 488, 10);
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% channels{4} = setPrintName(channels{4}, 'FSC');
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% channels{4} = setLineSpec(channels{4}, 'k');
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% If the name is FSC or SSC (or one of those with '-A', '-H', or '-W') it will automatically be unprocessed; otherwise, set it
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% channels{4} = setIsUnprocessed(channels{4}, true);
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@@ -56,8 +56,8 @@
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% Size bead files are used for processing FSC-A units into um equivalent diameter
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% They are optional, and will not be used if size bead file is not set
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% sizebeadfile = [];
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sizebeadfile = '../example_controls/180614_PPS6K_A02.fcs';
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sizebeadfile = [];
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% sizebeadfile = '../example_controls/180614_PPS6K_A02.fcs';
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CM = ColorModel(beadfile, blankfile, channels, colorfiles, colorpairfiles, sizebeadfile);
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CM=set_translation_plot(CM, true);
@@ -79,11 +79,11 @@
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CM=set_translation_channel_min(CM,[2,2,2]);
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% Configuration for size beads, if used
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TASBEConfig.set('sizebeads.beadModel','SpheroTech PPS-6K'); % Entry from BeadCatalog.xls matching your beads
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% TASBEConfig.set('sizebeads.beadModel','SpheroTech PPS-6K'); % Entry from BeadCatalog.xls matching your beads
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% Can also set bead channel or batch, if alternatives are available
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% Ignore all size bead data below 10^[rangeMin] as being too "smeared" with noise
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TASBEConfig.set('sizebeads.rangeMin', 2);
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CM=set_um_channel_name(CM, 'FSC-A');
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% TASBEConfig.set('sizebeads.rangeMin', 2);
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% CM=set_um_channel_name(CM, 'FSC-A');
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% When dealing with very strong fluorescence, use secondary channel to segment
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% TASBEConfig.set('beads.secondaryBeadChannel','PE-Tx-Red-YG-A');

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