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docs/source/conf.py

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# -- Project information -----------------------------------------------------
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project = "navigate"
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copyright = "2023, Dean Lab, UT Southwestern Medical Center"
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copyright = "2024, Dean Lab, UT Southwestern Medical Center"
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author = "Dean Lab, UT Southwestern Medical Center"
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# The full version, including alpha/beta/rc tags
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# -- LaTeX output options ----------------------------------------------------
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latex_elements = {'preamble': r'''
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latex_elements = {
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"preamble": r"""
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\usepackage[utf8]{inputenc}
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\usepackage{enumitem}
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\setlistdepth{99}
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\DeclareUnicodeCharacter{03BC}{$\mu$}
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''',
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'extraclassoptions': 'openany,oneside'}
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""",
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"extraclassoptions": "openany,oneside",
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}
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latex_documents = [
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('index', 'navigate.tex', 'navigate Documentation',
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'Dean Lab, UT Southwestern Medical Center', 'manual', True),
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(
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"index",
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"navigate.tex",
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"navigate Documentation",
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"Dean Lab, UT Southwestern Medical Center",
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"manual",
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True,
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),
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]
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latex_domain_indices = False
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latex_domain_indices = False

docs/source/implementations/implementations.rst

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@@ -51,6 +51,10 @@ quantitative imaging applications such as fluorescence resonance energy transfer
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single particle tracking. This combination of features makes ASLM an exceptional tool
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for detailed, quantitative imaging across large sample volumes.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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* `Deconvolution-free subcellular imaging with axially swept light sheet microscopy
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Images are captured using a Hamamatsu Flash 4.0 sCMOS camera. Multi-well plate plate imaging
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is available through mechanical sample scanning.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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* `Increasing the field-of-view in oblique plane microscopy via optical tiling
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objective, directed by a custom glass-tipped tertiary objective at a 40-degree angle, and
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finally captured on a Hamamatsu Flash 4.0 sCMOS camera.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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* `Adaptive optics in an oblique plane microscope <https://www.biorxiv.org/content/10.1101/2024.03.21.586191v1>`_
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wheel and 3D motorized stage from Sutter Instruments, which enables tiling of large volumes.
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For acquisition of Z-stacks, the microscope uses a piezo stage from Mad City Labs.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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Instruments 3D motorized stage, which supports the tiling of large volumes. Z-stacks
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are acquired with a 200-micron Piezosystem Jena stage.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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* `Isotropic imaging across spatial scales with axially swept light-sheet microscopy <https://www.nature.com/articles/s41596-022-00706-6>`_
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spatial context, significantly reducing the need for computational post-processing and
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eliminating data interpolation and duplication.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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* `Mechanically sheared Axially Swept Light-Sheet Microscopy <https://www.biorxiv.org/content/10.1101/2024.04.10.588892>`_
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------------------
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MCT ASLM
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MCT-ASLM
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********
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.. image:: mct_aslm.png
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quickly producing detailed scans of large cleared tissues (up to 20 x 20 x 20 mm in size)
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at a resolution of 5 microns. Furthermore, it integrates advanced computer vision techniques
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to automatically identify biological features of interest and allows for detailed
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analysis with isotropic resolution up to 300 nm.
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analysis with isotropic resolution up to 300 nm. A publication describing the MCT-ASLM
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system is currently in preparation.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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switching between epi-illumination and TIRF modes. This microscope supports FRET detection,
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expanding its utility in live cell imaging applications. Imaging is executed using an
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Olympus NA 1.7 TIRF lens (APON100XHOTIRF) paired with two Hamamatsu Flash 4.0 v3 cameras,
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enabling high-resolution and high-sensitivity imaging.
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enabling high-resolution and high-sensitivity imaging. A publication describing the
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spectral TIRF system is currently in preparation.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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scanned illumination and an electrotunable lens facilitates scanning of the illumination
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beam along its propagation direction, enabling Axially Swept Light-Sheet Imaging. Additionally,
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the setup includes a Meca500 robotic arm, which automates the loading and unloading of
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samples, significantly enhancing experimental throughput.
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samples, significantly enhancing experimental throughput. A publication describing the
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robotic mesoSPIM system is currently in preparation.
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**Instrument Location**:
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The University of Texas Southwestern Medical Center, Dallas, TX
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**Relevant Publications**:
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Understanding the mechanisms of neural tube closure is crucial, as its failure leads to
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neural tube defects such as spina bifida.
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**Instrument Location**:
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The University of Colorado, Boulder, CO
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**Relevant Publications**:
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* `A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
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|
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------------------
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Shepherd Lab MesoSPIM
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**********************
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More coming soon.
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**Instrument Location**:
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Arizona State University, Tempe, AZ
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**Relevant Publications**:
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* `The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue <https://www.nature.com/articles/s41592-019-0554-0>`_.
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* `Isotropic imaging across spatial scales with axially swept light-sheet microscopy <https://www.nature.com/articles/s41596-022-00706-6>`_
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* `Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution <https://www.nature.com/articles/s41592-019-0615-4>`_.
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.. collapse:: Technical Information
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.. list-table::
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:widths: 25 75
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:header-rows: 1
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* - Equipment
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- Description
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* - Lasers
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- TBD
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* - Stages
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- TBD
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* - Stage Controllers
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- TBD
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* - Cameras
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- TBD
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* - Filter Wheel
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- TBD
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* - Remote Focusing Units
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- TBD
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* - Data Acquisition Cards
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- TBD
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* - Galvo
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- TBD
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* - Zoom
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- TBD
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* - Other
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- TBD
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------------------
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Shepherd Lab OPM
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**********************
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More coming soon.
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**Instrument Location**:
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Arizona State University, Tempe, AZ
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**Relevant Publications**:
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* `Increasing the field-of-view in oblique plane microscopy via optical tiling
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<https://opg.optica.org/abstract.cfm?uri=boe-13-11-5616>`_
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* `A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
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<https://elifesciences.org/articles/57681>`_
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.. collapse:: Technical Information
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.. list-table::
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:widths: 25 75
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:header-rows: 1
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* - Equipment
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- Description
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* - Lasers
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- TBD
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* - Stages
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- TBD
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* - Stage Controllers
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- TBD
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* - Cameras
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- TBD
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* - Filter Wheel
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- TBD
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* - Remote Focusing Units
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- TBD
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* - Data Acquisition Cards
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- TBD
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* - Galvo
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- TBD
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* - Zoom
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- TBD
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* - Other
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- TBD

docs/source/index.rst

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**Project Philosophy**
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=========================
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* Prioritize standard library imports for maximum stability, ahd minimize external dependencies.
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* Prioritize standard library imports for maximum stability, and minimize external
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dependencies.
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* Abstraction layer to drive different camera types, etc.
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* Plugin architecture for extensibility.
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* Maximize productivity for biological users through robust graphical user interface-based workflows.

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