@@ -51,6 +51,10 @@ quantitative imaging applications such as fluorescence resonance energy transfer
5151single particle tracking. This combination of features makes ASLM an exceptional tool
5252for detailed, quantitative imaging across large sample volumes.
5353
54+ **Instrument Location **:
55+
56+ The University of Texas Southwestern Medical Center, Dallas, TX
57+
5458**Relevant Publications **:
5559
5660* `Deconvolution-free subcellular imaging with axially swept light sheet microscopy
@@ -131,6 +135,10 @@ and redirected by a custom glass-tipped tertiary objective set at a 40-degree an
131135Images are captured using a Hamamatsu Flash 4.0 sCMOS camera. Multi-well plate plate imaging
132136is available through mechanical sample scanning.
133137
138+ **Instrument Location **:
139+
140+ The University of Texas Southwestern Medical Center, Dallas, TX
141+
134142**Relevant Publications **:
135143
136144* `Increasing the field-of-view in oblique plane microscopy via optical tiling
@@ -201,6 +209,10 @@ objective in an epi-fluorescence format. The signal is then relayed through an O
201209objective, directed by a custom glass-tipped tertiary objective at a 40-degree angle, and
202210finally captured on a Hamamatsu Flash 4.0 sCMOS camera.
203211
212+ **Instrument Location **:
213+
214+ The University of Texas Southwestern Medical Center, Dallas, TX
215+
204216**Relevant Publications **:
205217
206218* `Adaptive optics in an oblique plane microscope <https://www.biorxiv.org/content/10.1101/2024.03.21.586191v1 >`_
@@ -268,6 +280,9 @@ with a Hamamatsu ORCA Flash 4.0 sCMOS camera. It is also equipped with a fast fi
268280wheel and 3D motorized stage from Sutter Instruments, which enables tiling of large volumes.
269281For acquisition of Z-stacks, the microscope uses a piezo stage from Mad City Labs.
270282
283+ **Instrument Location **:
284+
285+ The University of Texas Southwestern Medical Center, Dallas, TX
271286
272287**Relevant Publications **:
273288
@@ -330,6 +345,10 @@ from Applied Scientific Instrumentation. Sample positioning is handled by a Sutt
330345Instruments 3D motorized stage, which supports the tiling of large volumes. Z-stacks
331346are acquired with a 200-micron Piezosystem Jena stage.
332347
348+ **Instrument Location **:
349+
350+ The University of Texas Southwestern Medical Center, Dallas, TX
351+
333352**Relevant Publications **:
334353
335354* `Isotropic imaging across spatial scales with axially swept light-sheet microscopy <https://www.nature.com/articles/s41596-022-00706-6 >`_
@@ -403,6 +422,10 @@ are scanned simultaneously. This approach aligns data automatically in its corr
403422spatial context, significantly reducing the need for computational post-processing and
404423eliminating data interpolation and duplication.
405424
425+ **Instrument Location **:
426+
427+ The University of Texas Southwestern Medical Center, Dallas, TX
428+
406429**Relevant Publications **:
407430
408431* `Mechanically sheared Axially Swept Light-Sheet Microscopy <https://www.biorxiv.org/content/10.1101/2024.04.10.588892 >`_
@@ -448,7 +471,7 @@ eliminating data interpolation and duplication.
448471------------------
449472
450473
451- MCT ASLM
474+ MCT- ASLM
452475********
453476
454477.. image :: mct_aslm.png
@@ -490,7 +513,12 @@ switching between macroscale and nanoscale imaging modes. The MCT-ASLM is adept
490513quickly producing detailed scans of large cleared tissues (up to 20 x 20 x 20 mm in size)
491514at a resolution of 5 microns. Furthermore, it integrates advanced computer vision techniques
492515to automatically identify biological features of interest and allows for detailed
493- analysis with isotropic resolution up to 300 nm.
516+ analysis with isotropic resolution up to 300 nm. A publication describing the MCT-ASLM
517+ system is currently in preparation.
518+
519+ **Instrument Location **:
520+
521+ The University of Texas Southwestern Medical Center, Dallas, TX
494522
495523**Relevant Publications **:
496524
@@ -554,7 +582,12 @@ illumination across the field of view and allows for rapid, wavelength-dependent
554582switching between epi-illumination and TIRF modes. This microscope supports FRET detection,
555583expanding its utility in live cell imaging applications. Imaging is executed using an
556584Olympus NA 1.7 TIRF lens (APON100XHOTIRF) paired with two Hamamatsu Flash 4.0 v3 cameras,
557- enabling high-resolution and high-sensitivity imaging.
585+ enabling high-resolution and high-sensitivity imaging. A publication describing the
586+ spectral TIRF system is currently in preparation.
587+
588+ **Instrument Location **:
589+
590+ The University of Texas Southwestern Medical Center, Dallas, TX
558591
559592**Relevant Publications **:
560593
@@ -618,7 +651,12 @@ camera and an 8-position emission filter wheel. A galvanometer provides digitall
618651scanned illumination and an electrotunable lens facilitates scanning of the illumination
619652beam along its propagation direction, enabling Axially Swept Light-Sheet Imaging. Additionally,
620653the setup includes a Meca500 robotic arm, which automates the loading and unloading of
621- samples, significantly enhancing experimental throughput.
654+ samples, significantly enhancing experimental throughput. A publication describing the
655+ robotic mesoSPIM system is currently in preparation.
656+
657+ **Instrument Location **:
658+
659+ The University of Texas Southwestern Medical Center, Dallas, TX
622660
623661**Relevant Publications **:
624662
@@ -677,6 +715,10 @@ embryonic structure that eventually forms the brain and spinal cord.
677715Understanding the mechanisms of neural tube closure is crucial, as its failure leads to
678716neural tube defects such as spina bifida.
679717
718+ **Instrument Location **:
719+
720+ The University of Colorado, Boulder, CO
721+
680722**Relevant Publications **:
681723
682724* `A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
@@ -720,3 +762,97 @@ neural tube defects such as spina bifida.
720762 |
721763
722764------------------
765+
766+ Shepherd Lab MesoSPIM
767+ **********************
768+
769+ More coming soon.
770+
771+ **Instrument Location **:
772+
773+ Arizona State University, Tempe, AZ
774+
775+ **Relevant Publications **:
776+
777+ * `The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue <https://www.nature.com/articles/s41592-019-0554-0 >`_.
778+ * `Isotropic imaging across spatial scales with axially swept light-sheet microscopy <https://www.nature.com/articles/s41596-022-00706-6 >`_
779+ * `Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution <https://www.nature.com/articles/s41592-019-0615-4 >`_.
780+
781+
782+ .. collapse :: Technical Information
783+
784+ .. list-table ::
785+ :widths: 25 75
786+ :header-rows: 1
787+
788+ * - Equipment
789+ - Description
790+ * - Lasers
791+ - TBD
792+ * - Stages
793+ - TBD
794+ * - Stage Controllers
795+ - TBD
796+ * - Cameras
797+ - TBD
798+ * - Filter Wheel
799+ - TBD
800+ * - Remote Focusing Units
801+ - TBD
802+ * - Data Acquisition Cards
803+ - TBD
804+ * - Galvo
805+ - TBD
806+ * - Zoom
807+ - TBD
808+ * - Other
809+ - TBD
810+
811+
812+
813+ ------------------
814+
815+ Shepherd Lab OPM
816+ **********************
817+
818+ More coming soon.
819+
820+ **Instrument Location **:
821+
822+ Arizona State University, Tempe, AZ
823+
824+ **Relevant Publications **:
825+
826+ * `Increasing the field-of-view in oblique plane microscopy via optical tiling
827+ <https://opg.optica.org/abstract.cfm?uri=boe-13-11-5616> `_
828+ * `A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics
829+ <https://elifesciences.org/articles/57681> `_
830+
831+ .. collapse :: Technical Information
832+
833+ .. list-table ::
834+ :widths: 25 75
835+ :header-rows: 1
836+
837+ * - Equipment
838+ - Description
839+ * - Lasers
840+ - TBD
841+ * - Stages
842+ - TBD
843+ * - Stage Controllers
844+ - TBD
845+ * - Cameras
846+ - TBD
847+ * - Filter Wheel
848+ - TBD
849+ * - Remote Focusing Units
850+ - TBD
851+ * - Data Acquisition Cards
852+ - TBD
853+ * - Galvo
854+ - TBD
855+ * - Zoom
856+ - TBD
857+ * - Other
858+ - TBD
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