What is "effective NA" in the software

[GalaxyNight-day]

First of all, congratz on new paper (SACD)! I tried it, and it works really well :)

Question: what is "effective NA" in the sparse-sim software? if I were to use confocal with 1.27NA water lens, for example, what value should I put in...?

Thank you very much!

[WeisongZhao]

Hi,
Thanks a lot for your kind words :).

The effective NA represents the effective resolution of a system (wavelength/2NA), and it is adjustable in a reasonable range. For example of SIM, we usually use 3 as effective NA for 1.7 (objective NA) + 1.3 (illumination NA).

If you are using a confocal microscope without a super-resolution module, you can use the objective NA directly.