== Error == system call for: "['...']" finished abnormally, OS return value: 2

[TX0814]

Hi!
I'm running metaspades but it failed.
== Running: /home/cloudam/.conda/envs/spades/bin/spades-core /home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/config.info /home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/mda_mode.info /home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/meta_mode.info

0:00:00.000 1M / 23M INFO General (main.cpp : 99) Loaded config from /home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/config.info
0:00:00.007 1M / 23M INFO General (main.cpp : 99) Loaded config from /home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/mda_mode.info
0:00:00.008 1M / 23M INFO General (main.cpp : 99) Loaded config from /home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/meta_mode.info
0:00:00.009 1M / 23M INFO General (memory_limit.cpp : 54) Memory limit set to 128 Gb
0:00:00.009 1M / 23M INFO General (main.cpp : 107) Starting SPAdes, built from N/A, git revision N/A
0:00:00.009 1M / 23M INFO General (main.cpp : 108) Maximum k-mer length: 128
0:00:00.009 1M / 23M INFO General (main.cpp : 109) Assembling dataset (/home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/dataset.info) with K=21
0:00:00.009 1M / 23M INFO General (main.cpp : 110) Maximum # of threads to use (adjusted due to OMP capabilities): 16
0:00:00.009 1M / 23M INFO General (pipeline.cpp : 212) SPAdes started
0:00:00.009 1M / 23M ERROR General (pipeline.cpp : 216) Sorry, current version of metaSPAdes can work either with single library (paired-end only) or in hybrid paired-end + (TSLR or PacBio or Nanopore) mode.

== Error == system call for: "['/home/cloudam/.conda/envs/spades/bin/spades-core', '/home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/config.info', '/home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/mda_mode.info', '/home/cloudam/raw_data/tx/DAMO_Bacteria_raw/genome/K21/configs/meta_mode.info']" finished abnormally, OS return value: 2
None

Here are my spades.log and params.txt files from this run:
spades.log
params.txt

[asl]

The log clearly reads:

Sorry, current version of metaSPAdes can work either with single library (paired-end only) or in hybrid paired-end + (TSLR or PacBio or Nanopore) mode.

[TX0814]

So, does it mean that I should merge the forward and reverse of paired-end only into a single library and then use metaSPAdes?

[asl]

If your libraries are "compatible" (e.g have similar fragment length distribution) then this might work