diff --git a/drivers/i2c/i2c_dw.c b/drivers/i2c/i2c_dw.c
index ef2b205ab9c..0edcd147052 100644
--- a/drivers/i2c/i2c_dw.c
+++ b/drivers/i2c/i2c_dw.c
@@ -541,12 +541,17 @@ static void i2c_dw_isr(const struct device *port)
 				}
 			}
 #else
-			if (slave_cb->read_requested) {
-				slave_cb->read_requested(dw->slave_cfg, &data);
-				i2c_dw_write_byte_non_blocking(port, data);
-			}
-			if (slave_cb->read_processed) {
-				slave_cb->read_processed(dw->slave_cfg, &data);
+			if (!dw->read_in_progress) {
+				if (slave_cb->read_requested) {
+					slave_cb->read_requested(dw->slave_cfg, &data);
+					i2c_dw_write_byte_non_blocking(port, data);
+				}
+				dw->read_in_progress = true;
+			} else {
+				if (slave_cb->read_processed) {
+					slave_cb->read_processed(dw->slave_cfg, &data);
+					i2c_dw_write_byte_non_blocking(port, data);
+				}
 			}
 #endif
 		}
@@ -986,6 +991,7 @@ static int i2c_dw_slave_register(const struct device *dev, struct i2c_target_con
 	uint32_t reg_base = get_regs(dev);
 	int ret;
 
+	dw->read_in_progress = false;
 	dw->slave_cfg = cfg;
 	ret = i2c_dw_set_slave_mode(dev, cfg->address, cfg->flags);
 	write_intr_mask(DW_INTR_MASK_RX_FULL | DW_INTR_MASK_RD_REQ | DW_INTR_MASK_TX_ABRT |
@@ -1049,6 +1055,7 @@ static void i2c_dw_slave_read_clear_intr_bits(const struct device *dev)
 	if (intr_stat.bits.stop_det) {
 		read_clr_stop_det(reg_base);
 		dw->state = I2C_DW_STATE_READY;
+		dw->read_in_progress = false;
 		if (slave_cb->stop) {
 			slave_cb->stop(dw->slave_cfg);
 		}
@@ -1057,6 +1064,7 @@ static void i2c_dw_slave_read_clear_intr_bits(const struct device *dev)
 	if (intr_stat.bits.start_det) {
 		read_clr_start_det(reg_base);
 		dw->state = I2C_DW_STATE_READY;
+		dw->read_in_progress = false;
 	}
 
 	if (intr_stat.bits.gen_call) {
diff --git a/drivers/i2c/i2c_dw.h b/drivers/i2c/i2c_dw.h
index 57c2dd607fd..ed763820725 100644
--- a/drivers/i2c/i2c_dw.h
+++ b/drivers/i2c/i2c_dw.h
@@ -173,6 +173,7 @@ struct i2c_dw_dev_config {
 	uint32_t request_bytes;
 	uint8_t xfr_flags;
 	bool support_hs_mode;
+	bool read_in_progress;
 #ifdef CONFIG_I2C_DW_LPSS_DMA
 	uintptr_t phy_addr;
 	uintptr_t base_addr;