diff --git a/pipeline_consensus/Snakefile b/pipeline_consensus/Snakefile index 0cc0566f..66df46e4 100644 --- a/pipeline_consensus/Snakefile +++ b/pipeline_consensus/Snakefile @@ -31,7 +31,7 @@ rule call_consensus: "{out_dir}/consensus_sequences/{sample}.fa" shell: """ - samtools mpileup -A -Q 0 -d 0 {input} | ivar consensus -p {output} -m 10 -n N + samtools mpileup -A -a -Q 0 -d 0 {input} | ivar consensus -p {output} -m 10 -n N """ rule trim_reads: