Using this image with tile size as 16x16 and sprite sheet width of 256

The resulting tilemap has a black pixel in it, which is 0 in the png data at (50,29)

Here is a scaled copy of the tilemap at 4x to help show the black pixel

Third row up from bottom, about a third of the way across.
I am digging into this now and see if I can figure it out. But I figured I'd post here too in case this is known or anyone has any ideas.
If I figure it out, I'll submit a PR.
Oh and here is the tileset, in case that is helpful
