diff --git a/.gitignore b/.gitignore
index feb3ede744a..33a707d8cc6 100644
--- a/.gitignore
+++ b/.gitignore
@@ -11,6 +11,7 @@ packages/**/esm/
 packages/**/cjs/
 **/.DS_Store
 .vscode
+.kiro
 .idea
 *.log
 .npm/
diff --git a/packages/core/__tests__/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.test.ts b/packages/core/__tests__/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.test.ts
index db7289da109..ec9914bdea3 100644
--- a/packages/core/__tests__/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.test.ts
+++ b/packages/core/__tests__/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.test.ts
@@ -2,6 +2,7 @@
 // SPDX-License-Identifier: Apache-2.0
 
 import { getHashedPayload } from '../../../../../../../src/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload';
+import { UNSIGNED_PAYLOAD } from '../../../../../../../src/clients/middleware/signing/signer/signatureV4/constants';
 
 describe('getHashedPayload', () => {
 	test('returns empty hash if nullish', () => {
@@ -34,4 +35,10 @@ describe('getHashedPayload', () => {
 			expect(getHashedPayload(scalar as any)).toStrictEqual('UNSIGNED-PAYLOAD');
 		}
 	});
+
+	test('returns UNSIGNED_PAYLOAD constant without hashing when passed as body', () => {
+		expect(getHashedPayload(UNSIGNED_PAYLOAD as any)).toStrictEqual(
+			'UNSIGNED-PAYLOAD',
+		);
+	});
 });
diff --git a/packages/core/src/clients/index.ts b/packages/core/src/clients/index.ts
index f1c4d671223..743d62cdc5b 100644
--- a/packages/core/src/clients/index.ts
+++ b/packages/core/src/clients/index.ts
@@ -13,7 +13,10 @@ export {
 	PresignUrlOptions,
 	SignRequestOptions,
 } from './middleware/signing/signer/signatureV4';
-export { EMPTY_HASH as EMPTY_SHA256_HASH } from './middleware/signing/signer/signatureV4/constants';
+export {
+	EMPTY_HASH as EMPTY_SHA256_HASH,
+	UNSIGNED_PAYLOAD,
+} from './middleware/signing/signer/signatureV4/constants';
 export { extendedEncodeURIComponent } from './middleware/signing/utils/extendedEncodeURIComponent';
 export {
 	signingMiddlewareFactory,
diff --git a/packages/core/src/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.ts b/packages/core/src/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.ts
index 8fa93dc2136..7dd22cdc0bf 100644
--- a/packages/core/src/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.ts
+++ b/packages/core/src/clients/middleware/signing/signer/signatureV4/utils/getHashedPayload.ts
@@ -34,7 +34,9 @@ export const getHashedPayload = (body: HttpRequest['body']): string => {
 };
 
 const isSourceData = (body: HttpRequest['body']): body is SourceData =>
-	typeof body === 'string' || ArrayBuffer.isView(body) || isArrayBuffer(body);
+	// Exclude UNSIGNED_PAYLOAD constant to prevent it from being hashed as a string
+	body !== UNSIGNED_PAYLOAD &&
+	(typeof body === 'string' || ArrayBuffer.isView(body) || isArrayBuffer(body));
 
 const isArrayBuffer = (arg: any): arg is ArrayBuffer =>
 	(typeof ArrayBuffer === 'function' && arg instanceof ArrayBuffer) ||
diff --git a/packages/storage/__tests__/providers/s3/utils/client/S3/getPresignedGetObjectUrl.test.ts b/packages/storage/__tests__/providers/s3/utils/client/S3/getPresignedGetObjectUrl.test.ts
index a208859a7c8..403a6162010 100644
--- a/packages/storage/__tests__/providers/s3/utils/client/S3/getPresignedGetObjectUrl.test.ts
+++ b/packages/storage/__tests__/providers/s3/utils/client/S3/getPresignedGetObjectUrl.test.ts
@@ -1,7 +1,10 @@
 // Copyright Amazon.com, Inc. or its affiliates. All Rights Reserved.
 // SPDX-License-Identifier: Apache-2.0
 
-import { presignUrl } from '@aws-amplify/core/internals/aws-client-utils';
+import {
+	UNSIGNED_PAYLOAD,
+	presignUrl,
+} from '@aws-amplify/core/internals/aws-client-utils';
 
 import { getPresignedGetObjectUrl } from '../../../../../../src/providers/s3/utils/client/s3data';
 
@@ -42,9 +45,6 @@ describe('serializeGetObjectRequest', () => {
 		);
 		expect(actualUrl.pathname).toEqual('/key');
 		expect(actualUrl.searchParams.get('X-Amz-Expires')).toEqual('900');
-		expect(actualUrl.searchParams.get('x-amz-content-sha256')).toEqual(
-			expect.any(String),
-		);
 		expect(actualUrl.searchParams.get('x-amz-user-agent')).toEqual('UA');
 	});
 
@@ -99,9 +99,6 @@ describe('serializeGetObjectRequest', () => {
 		);
 
 		expect(actual.searchParams.get('X-Amz-Expires')).toBe('900');
-		expect(actual.searchParams.get('x-amz-content-sha256')).toEqual(
-			expect.any(String),
-		);
 		expect(actual.searchParams.get('response-content-disposition')).toBe(
 			'attachment; filename="filename.jpg"',
 		);
@@ -110,4 +107,32 @@ describe('serializeGetObjectRequest', () => {
 		);
 		expect(actual.searchParams.get('x-amz-user-agent')).toBe('UA');
 	});
+
+	it('should use UNSIGNED-PAYLOAD for presigned URLs', async () => {
+		mockPresignUrl.mockClear();
+
+		const result = await getPresignedGetObjectUrl(
+			{
+				...defaultConfigWithStaticCredentials,
+				signingRegion: defaultConfigWithStaticCredentials.region,
+				signingService: 's3',
+				expiration: 900,
+			},
+			{
+				Bucket: 'bucket',
+				Key: 'key',
+			},
+		);
+
+		// Verify UNSIGNED-PAYLOAD is used in the signature calculation
+		expect(mockPresignUrl).toHaveBeenCalledWith(
+			expect.objectContaining({
+				body: UNSIGNED_PAYLOAD,
+			}),
+			expect.anything(),
+		);
+
+		// Verify x-amz-content-sha256 is NOT in the presigned URL query parameters
+		expect(result.searchParams.get('x-amz-content-sha256')).toBeNull();
+	});
 });
diff --git a/packages/storage/src/providers/s3/utils/client/s3data/getObject.ts b/packages/storage/src/providers/s3/utils/client/s3data/getObject.ts
index 40d8e067892..223c671cb58 100644
--- a/packages/storage/src/providers/s3/utils/client/s3data/getObject.ts
+++ b/packages/storage/src/providers/s3/utils/client/s3data/getObject.ts
@@ -2,11 +2,11 @@
 // SPDX-License-Identifier: Apache-2.0
 
 import {
-	EMPTY_SHA256_HASH,
 	Endpoint,
 	HttpRequest,
 	HttpResponse,
 	PresignUrlOptions,
+	UNSIGNED_PAYLOAD,
 	UserAgentOptions,
 	parseMetadata,
 	presignUrl,
@@ -18,7 +18,6 @@ import {
 } from '@aws-amplify/core/internals/utils';
 
 import {
-	CONTENT_SHA256_HEADER,
 	assignStringVariables,
 	buildStorageServiceError,
 	deserializeBoolean,
@@ -172,10 +171,6 @@ export const getPresignedGetObjectUrl = async (
 	const endpoint = defaultConfig.endpointResolver(config, input);
 	const { url, headers, method } = await getObjectSerializer(input, endpoint);
 
-	// TODO: set content sha256 query parameter with value of UNSIGNED-PAYLOAD instead of empty hash.
-	// It requires changes in presignUrl. Without this change, the generated url still works,
-	// but not the same as other tools like AWS SDK and CLI.
-	url.searchParams.append(CONTENT_SHA256_HEADER, EMPTY_SHA256_HASH);
 	if (config.userAgentValue) {
 		url.searchParams.append(
 			config.userAgentHeader ?? USER_AGENT_HEADER,
@@ -199,7 +194,7 @@ export const getPresignedGetObjectUrl = async (
 	}
 
 	return presignUrl(
-		{ method, url, body: undefined },
+		{ method, url, body: UNSIGNED_PAYLOAD },
 		{
 			signingService: defaultConfig.service,
 			signingRegion: config.region,