diff --git a/.github/workflows/java-sample-app-s3-deploy.yml b/.github/workflows/java-sample-app-s3-deploy.yml
index 371ed91e5..ba5a52137 100644
--- a/.github/workflows/java-sample-app-s3-deploy.yml
+++ b/.github/workflows/java-sample-app-s3-deploy.yml
@@ -160,4 +160,49 @@ jobs:
           gradle build -P javaVersion=${{ matrix.java-version }}
           aws s3api put-object --bucket aws-appsignals-sample-app-prod-us-east-1 --body ./build/libs/springboot-remote-service-*-SNAPSHOT.jar --key java-remote-service-v${{ matrix.java-version }}.jar
 
+  java-lambda:
+    strategy:
+      fail-fast: false
+      matrix:
+        aws-region: [ 'af-south-1','ap-east-1','ap-northeast-1','ap-northeast-2','ap-northeast-3','ap-south-1','ap-south-2','ap-southeast-1',
+                      'ap-southeast-2','ap-southeast-3','ap-southeast-4','ca-central-1','eu-central-1','eu-central-2','eu-north-1',
+                      'eu-south-1','eu-south-2','eu-west-1','eu-west-2','eu-west-3','il-central-1','me-central-1','me-south-1', 'sa-east-1',
+                      'us-east-1','us-east-2','us-west-1','us-west-2' ]
+    runs-on: ubuntu-latest
+    steps:
+      - name: Check out ADOT Java repo
+        uses: actions/checkout@v4
+        with:
+          repository: 'aws-observability/aws-otel-java-instrumentation'
+
+      - name: Setup Java
+        uses: actions/setup-java@v4
+        with:
+          distribution: 'temurin'
+          java-version: '17'
+
+      - name: Configure AWS Credentials
+        uses: aws-actions/configure-aws-credentials@v4
+        with:
+          role-to-assume: arn:aws:iam::${{ env.E2E_TEST_ACCOUNT_ID }}:role/${{ env.E2E_TEST_ROLE_NAME }}
+          aws-region: us-east-1
+
+      - name: Retrieve account
+        uses: aws-actions/aws-secretsmanager-get-secrets@v1
+        with:
+          secret-ids:
+            ACCOUNT_ID, region-account/${{ matrix.aws-region }}
+
+      - name: Configure AWS Credentials
+        uses: aws-actions/configure-aws-credentials@v4
+        with:
+          role-to-assume: arn:aws:iam::${{ env.ACCOUNT_ID }}:role/${{ env.E2E_TEST_ROLE_NAME }}
+          aws-region: ${{ matrix.aws-region }}
 
+      - name: Build, zip, and upload the sample app to S3
+        working-directory: sample-apps/apigateway-lambda
+        shell: bash
+        run: |
+          gradle build
+          gradle createLambdaZip
+          aws s3api put-object --bucket aws-appsignals-sample-app-prod-${{ matrix.aws-region }} --body ./build/distributions/lambda-function.zip --key java-lambda-function.zip