diff --git a/aws-distro-opentelemetry-node-autoinstrumentation/src/register.ts b/aws-distro-opentelemetry-node-autoinstrumentation/src/register.ts
index 8b9f1050..d3d0b2fa 100644
--- a/aws-distro-opentelemetry-node-autoinstrumentation/src/register.ts
+++ b/aws-distro-opentelemetry-node-autoinstrumentation/src/register.ts
@@ -47,19 +47,19 @@ export function setAwsDefaultEnvironmentVariables() {
     process.env.OTEL_PROPAGATORS = 'baggage,xray,tracecontext';
   }
   if (!process.env.OTEL_NODE_DISABLED_INSTRUMENTATIONS) {
-    if (isAgentObservabilityEnabled()) {
-      // Assume users only need instrumentations that are manually set-up outside of OpenTelemetry
-      process.env.OTEL_NODE_DISABLED_INSTRUMENTATIONS =
-        'amqplib,aws-lambda,aws-sdk,bunyan,cassandra-driver,connect,cucumber,dataloader,dns,express,fastify,fs,generic-pool,graphql,grpc,hapi,http,ioredis,kafkajs,knex,koa,lru-memoizer,memcached,mongodb,mongoose,mysql2,mysql,nestjs-core,net,pg,pino,redis,redis-4,restify,router,socket.io,tedious,undici,winston';
-    } else {
-      // Disable the following instrumentations by default
-      // This auto-instrumentation for the `fs` module generates many low-value spans. `dns` is similar.
-      // https://github.com/open-telemetry/opentelemetry-js-contrib/issues/1344#issuecomment-1618993178
-      process.env.OTEL_NODE_DISABLED_INSTRUMENTATIONS = 'fs,dns';
-    }
+    // Disable the following instrumentations by default
+    // This auto-instrumentation for the `fs` module generates many low-value spans. `dns` is similar.
+    // https://github.com/open-telemetry/opentelemetry-js-contrib/issues/1344#issuecomment-1618993178
+    process.env.OTEL_NODE_DISABLED_INSTRUMENTATIONS = 'fs,dns';
   }
 
   if (isAgentObservabilityEnabled()) {
+    if (!process.env.OTEL_NODE_ENABLED_INSTRUMENTATIONS) {
+      // Assume users only need aws-sdk and aws-lambda instrumentations, as well as
+      // instrumentations that are manually set-up outside of OpenTelemetry
+      process.env.OTEL_NODE_ENABLED_INSTRUMENTATIONS = 'aws-lambda,aws-sdk';
+    }
+
     // Set exporter defaults
     if (!process.env.OTEL_TRACES_EXPORTER) {
       process.env.OTEL_TRACES_EXPORTER = 'otlp';
diff --git a/aws-distro-opentelemetry-node-autoinstrumentation/test/register.test.ts b/aws-distro-opentelemetry-node-autoinstrumentation/test/register.test.ts
index c2358ac9..47e4d5ac 100644
--- a/aws-distro-opentelemetry-node-autoinstrumentation/test/register.test.ts
+++ b/aws-distro-opentelemetry-node-autoinstrumentation/test/register.test.ts
@@ -28,6 +28,7 @@ describe('Register', function () {
       delete process.env.OTEL_EXPORTER_OTLP_PROTOCOL;
       delete process.env.OTEL_PROPAGATORS;
       delete process.env.OTEL_NODE_DISABLED_INSTRUMENTATIONS;
+      delete process.env.OTEL_NODE_ENABLED_INSTRUMENTATIONS;
 
       delete process.env.AWS_REGION;
       delete process.env.AWS_DEFAULT_REGION;
@@ -100,9 +101,8 @@ describe('Register', function () {
       expect(process.env.OTEL_METRICS_EXPORTER).toEqual('awsemf');
       expect(process.env.OTEL_INSTRUMENTATION_GENAI_CAPTURE_MESSAGE_CONTENT).toEqual('true');
       expect(process.env.OTEL_TRACES_SAMPLER).toEqual('parentbased_always_on');
-      expect(process.env.OTEL_NODE_DISABLED_INSTRUMENTATIONS).toEqual(
-        'amqplib,aws-lambda,aws-sdk,bunyan,cassandra-driver,connect,cucumber,dataloader,dns,express,fastify,fs,generic-pool,graphql,grpc,hapi,http,ioredis,kafkajs,knex,koa,lru-memoizer,memcached,mongodb,mongoose,mysql2,mysql,nestjs-core,net,pg,pino,redis,redis-4,restify,router,socket.io,tedious,undici,winston'
-      );
+      expect(process.env.OTEL_NODE_DISABLED_INSTRUMENTATIONS).toEqual('fs,dns');
+      expect(process.env.OTEL_NODE_ENABLED_INSTRUMENTATIONS).toEqual('aws-lambda,aws-sdk');
       expect(process.env.OTEL_AWS_APPLICATION_SIGNALS_ENABLED).toEqual('false');
       expect(process.env.OTEL_EXPORTER_OTLP_TRACES_ENDPOINT).toEqual('https://xray.us-east-1.amazonaws.com/v1/traces');
       expect(process.env.OTEL_EXPORTER_OTLP_LOGS_ENDPOINT).toEqual('https://logs.us-east-1.amazonaws.com/v1/logs');