diff --git a/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/_aws_attribute_keys.py b/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/_aws_attribute_keys.py
index 923025894..a2b7cd00b 100644
--- a/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/_aws_attribute_keys.py
+++ b/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/_aws_attribute_keys.py
@@ -11,7 +11,7 @@
 AWS_REMOTE_RESOURCE_IDENTIFIER: str = "aws.remote.resource.identifier"
 AWS_SDK_DESCENDANT: str = "aws.sdk.descendant"
 AWS_CONSUMER_PARENT_SPAN_KIND: str = "aws.consumer.parent.span.kind"
-AWS_TRACE_FLAG_UNSAMPLED: str = "aws.trace.flag.unsampled"
+AWS_TRACE_FLAG_SAMPLED: str = "aws.trace.flag.sampled"
 
 # AWS_#_NAME attributes are not supported in python as they are not part of the Semantic Conventions.
 # TODO：Move to Semantic Conventions when these attributes are added.
diff --git a/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/aws_batch_unsampled_span_processor.py b/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/aws_batch_unsampled_span_processor.py
index 36e239188..367ff38ee 100644
--- a/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/aws_batch_unsampled_span_processor.py
+++ b/aws-opentelemetry-distro/src/amazon/opentelemetry/distro/aws_batch_unsampled_span_processor.py
@@ -3,22 +3,20 @@
 import logging
 from typing import Optional
 
-from amazon.opentelemetry.distro._aws_attribute_keys import AWS_TRACE_FLAG_UNSAMPLED
+from amazon.opentelemetry.distro._aws_attribute_keys import AWS_TRACE_FLAG_SAMPLED
 from opentelemetry.context import Context
 from opentelemetry.sdk.trace import ReadableSpan, Span
 from opentelemetry.sdk.trace.export import BatchSpanProcessor as BaseBatchSpanProcessor
 
 logger = logging.getLogger(__name__)
 
-SPANUNSAMPLED_FLAG = "OTEL_AWS_APP_SIGNALS_ENABLED"
-
 
 class BatchUnsampledSpanProcessor(BaseBatchSpanProcessor):
 
     # pylint: disable=no-self-use
     def on_start(self, span: Span, parent_context: Optional[Context] = None) -> None:
         if not span.context.trace_flags.sampled:
-            span.set_attribute(AWS_TRACE_FLAG_UNSAMPLED, True)
+            span.set_attribute(AWS_TRACE_FLAG_SAMPLED, False)
 
     def on_end(self, span: ReadableSpan) -> None:
         if span.context.trace_flags.sampled:
diff --git a/aws-opentelemetry-distro/tests/amazon/opentelemetry/distro/test_aws_batch_unsampled_span_processor.py b/aws-opentelemetry-distro/tests/amazon/opentelemetry/distro/test_aws_batch_unsampled_span_processor.py
index 004d2f1d1..5b6fed42e 100644
--- a/aws-opentelemetry-distro/tests/amazon/opentelemetry/distro/test_aws_batch_unsampled_span_processor.py
+++ b/aws-opentelemetry-distro/tests/amazon/opentelemetry/distro/test_aws_batch_unsampled_span_processor.py
@@ -3,7 +3,7 @@
 from unittest import TestCase
 from unittest.mock import MagicMock, patch
 
-from amazon.opentelemetry.distro._aws_attribute_keys import AWS_TRACE_FLAG_UNSAMPLED
+from amazon.opentelemetry.distro._aws_attribute_keys import AWS_TRACE_FLAG_SAMPLED
 from amazon.opentelemetry.distro.aws_batch_unsampled_span_processor import BatchUnsampledSpanProcessor
 from opentelemetry.trace import TraceFlags
 
@@ -43,7 +43,7 @@ def test_on_end_not_sampled(self, mock_span_class):
         self.processor.on_end(mock_span2)
 
         self.assertEqual(len(self.processor.queue), 1)
-        self.assertIn(AWS_TRACE_FLAG_UNSAMPLED, mock_span1.set_attribute.call_args_list[0][0][0])
+        self.assertIn(AWS_TRACE_FLAG_SAMPLED, mock_span1.set_attribute.call_args_list[0][0][0])
 
         self.processor.shutdown()
         mock_span2 = mock_span_class.return_value
diff --git a/lambda-layer/README.md b/lambda-layer/README.md
index dbdba611c..5eb0fab46 100644
--- a/lambda-layer/README.md
+++ b/lambda-layer/README.md
@@ -47,3 +47,10 @@ cd lambda-layer
 
 Once the script has successfully run, you will see the deployed Lambda sample app in your AWS account. You can trigger the 
 Lambda function and view the traces and metrics through the AWS CloudWatch Console.
+
+## Configuration
+
+By default the layer enable botocore and aws-lambda instrumentation libraries only for better Lambda cold start performance. To 
+enable all opentelemetry python 
+supported libraries you can set environment variable `OTEL_PYTHON_DISABLED_INSTRUMENTATIONS=none`. Refer to details in 
+[OpenTelemetry Python Disabling Specific Instrumentations](Disabling Specific Instrumentations)
\ No newline at end of file