diff --git a/.github/workflows/application-signals-e2e-test.yml b/.github/workflows/application-signals-e2e-test.yml
index 9fbf3a5fe..326e2fbc7 100644
--- a/.github/workflows/application-signals-e2e-test.yml
+++ b/.github/workflows/application-signals-e2e-test.yml
@@ -238,3 +238,12 @@ jobs:
     with:
       caller-workflow-name: 'main-build'
       staging-wheel-name: ${{ inputs.staging-wheel-name }}
+  
+  # Stand-Alone ADOT/ADOT Genesis test on EC2
+  adot-genesis:
+    needs: [ upload-main-build ]
+    uses: aws-observability/aws-application-signals-test-framework/.github/workflows/python-ec2-genesis-test.yml@main
+    secrets: inherit
+    with:
+      caller-workflow-name: 'main-build'
+      staging-wheel-name: ${{ inputs.staging-wheel-name }}