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| 1 | +Name: E11bio PRISM |
| 2 | +Description: | |
| 3 | + This dataset was generated using E11.bio's PRISM technology (Protein Reconstruction and Identification through Multiplexing), |
| 4 | + a platform that combines viral barcoding, expansion microscopy, and iterative immunolabeling for large-scale neuronal reconstruction. |
| 5 | +
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| 6 | + Neurons in the mouse hippocampal CA3 were transduced with a library of adeno-associated viruses (AAVs) |
| 7 | + encoding diverse “protein bits”—small epitope tags that act as combinatorial barcodes. |
| 8 | + Tissue was then processed with an expansion microscopy protocol, physically enlarging the sample ~5× |
| 9 | + to achieve an effective voxel size of ~35 × 35 × 80 nm. |
| 10 | + Across multiple cycles of staining, imaging, and antibody stripping, the same expanded tissue was repeatedly labeled, |
| 11 | + enabling iterative immunostaining for dozens of molecular targets. |
| 12 | +
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| 13 | + The dataset includes: |
| 14 | + 1) Light microscopy data of multiplexed brain tissue |
| 15 | + 2) Segmentations of cell morphology and protein expression in the tissue |
| 16 | + 3) Files for faster visualization of the data (e.g. precomputed format) |
| 17 | + 4) Additional supporting files (e.g. model predictions, manual annotations etc.) |
| 18 | +Documentation: https://github.com/e11bio/e11-open-data |
| 19 | + |
| 20 | +ManagedBy: "[E11.bio](https://e11.bio)" |
| 21 | +UpdateFrequency: As required |
| 22 | +Tags: |
| 23 | + - bioinformatics |
| 24 | + - biology |
| 25 | + - brain images |
| 26 | + - cell imaging |
| 27 | + - computer vision |
| 28 | + - fluorescence imaging |
| 29 | + - high-throughput imaging |
| 30 | + - image processing |
| 31 | + - imaging |
| 32 | + - ion channels |
| 33 | + - life sciences |
| 34 | + - machine learning |
| 35 | + - microscopy |
| 36 | + - morphological reconstructions |
| 37 | + - Mus musculus |
| 38 | + - neurobiology |
| 39 | + - neuroimaging |
| 40 | + - neuroscience |
| 41 | + - protein |
| 42 | + - segmentation |
| 43 | + - zarr |
| 44 | + - aws-pds |
| 45 | +License: https://e11.bio/terms-of-use |
| 46 | +Resources: |
| 47 | + - Description: Data files in a public bucket |
| 48 | + ARN: arn:aws:s3:::e11bio-prism |
| 49 | + Region: us-east-1 |
| 50 | + Type: S3 Bucket |
| 51 | +DataAtWork: |
| 52 | + Tutorials: |
| 53 | + - Title: E11.Bio PRISM OpenData |
| 54 | + URL: https://github.com/e11bio/e11-open-data |
| 55 | + NotebookURL: |
| 56 | + AuthorName: Arlo Sheridan & Johan Winnubst |
| 57 | + AuthorURL: https://e11.bio/team |
| 58 | + Tools & Applications: |
| 59 | + - Title: Volara |
| 60 | + URL: https://github.com/e11bio/volara |
| 61 | + AuthorName: Arlo Sheridan & Will Patton |
| 62 | + AuthorURL: https://e11.bio/team |
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